US 11,747,319 B2
Computer method and system for deriving cell-to-cell spatial proximities
Brian Little, Carlsbad, CA (US); and Jennifer Bordeaux, Carlsbad, CA (US)
Assigned to Novartis AG, Basel (CH)
Filed by Novartis AG, Basel (CH)
Filed on Feb. 14, 2022, as Appl. No. 17/671,501.
Application 17/671,501 is a continuation of application No. 16/898,416, filed on Jun. 10, 2020, granted, now 11,249,066.
Application 16/898,416 is a continuation of application No. 15/769,226, granted, now 10,712,331, previously published as PCT/US2016/058278, filed on Oct. 21, 2016.
Claims priority of provisional application 62/259,326, filed on Nov. 24, 2015.
Claims priority of provisional application 62/245,933, filed on Oct. 23, 2015.
Prior Publication US 2022/0170905 A1, Jun. 2, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/483 (2006.01); G01N 21/64 (2006.01); G01N 33/574 (2006.01); G01N 33/48 (2006.01); G16H 50/30 (2018.01); G16H 40/63 (2018.01); G16H 30/20 (2018.01); G06T 7/00 (2017.01)
CPC G01N 33/4833 (2013.01) [G01N 21/6456 (2013.01); G01N 33/48 (2013.01); G01N 33/57492 (2013.01); G06T 7/0012 (2013.01); G16H 30/20 (2018.01); G16H 40/63 (2018.01); G16H 50/30 (2018.01); G01N 21/6428 (2013.01); G01N 2021/6439 (2013.01); G01N 2800/52 (2013.01); G06T 2207/30096 (2013.01)] 20 Claims
OG exemplary drawing
 
9. A method of determining a score representative of a spatial proximity between at least one pair of cells in a tumor tissue sample taken from a cancer patient using an imaging system, the method comprising:
executing instructions stored on a computer-readable medium, wherein the instructions include:
receiving image data based on information about detected electromagnetic radiation captured at an imaging device;
analyzing the image data to determine the score;
wherein analyzing the image data comprises:
dilating fluorescence signals attributable to a first biomarker, expressed by a first member of the at least one pair of cells, by a predetermined margin that is selected to encompass a proximally located second member of the at least one pair of cells expressing a second biomarker that is different from the first biomarker;
determining an interaction area, which is a first total area for all cells which express the second biomarker and are encompassed within the dilated fluorescence signals attributable to the first biomarker;
calculating the score by dividing the interaction area by a normalization factor and multiplying the resulting quotient by a predetermined factor to arrive at a spatial proximity score.