CPC G01N 30/02 (2013.01) [B01D 15/305 (2013.01); C07D 215/48 (2013.01); C07H 15/26 (2013.01); G01N 30/04 (2013.01); G01N 30/88 (2013.01); G01N 33/6842 (2013.01); G01N 2030/027 (2013.01); G01N 2030/047 (2013.01); G01N 2030/626 (2013.01); G01N 2030/8836 (2013.01); G01N 2400/00 (2013.01); G01N 2440/38 (2013.01)] | 1 Claim |
1. A method of making a calibrant useful in liquid chromatography, comprising the steps of:
providing a reducing glycan having an aldehyde group;
reacting the aldehyde group of the reducing glycan with a compound having a primary amine through a reductive amination to produce an intermediary compound;
mixing the intermediary compound with a rapid tagging reagent, wherein the intermediary compound is rapidly labeled with the rapid tagging reagent; and
physically producing a rapid labeled dextran ladder having identical optical properties as a rapid labeled N-glycan wherein the rapid label N-glycan is produced by rapid tagging of a N-glycan with the rapid tagging reagent.
|