US 11,747,263 B2
Flow cells and methods related to same
Arnaud Rival, Saint Nazaire les Eymes (FR); Ali Agah, Menlo Park, CA (US); Tracy H. Fung, San Mateo, CA (US); Dietrich Dehlinger, San Francisco, CA (US); Poorya Sabounchi, Atherton, CA (US); Tarun Khurana, Fremont, CA (US); Craig M. Ciesla, Mountain View, CA (US); and M. Shane Bowen, Encinitas, CA (US)
Assigned to Illumina, Inc., San Diego, CA (US)
Filed by Illumina, Inc., San Diego, CA (US)
Filed on Jul. 11, 2022, as Appl. No. 17/811,750.
Application 17/811,750 is a continuation of application No. 17/148,214, filed on Jan. 13, 2021, granted, now 11,385,162.
Application 17/148,214 is a continuation of application No. 16/567,224, filed on Sep. 11, 2019, granted, now 10,921,233, issued on Feb. 16, 2021.
Claims priority of provisional application 62/731,785, filed on Sep. 14, 2018.
Prior Publication US 2022/0341837 A1, Oct. 27, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 15/14 (2006.01)
CPC G01N 15/1404 (2013.01) [G01N 15/1429 (2013.01); G01N 2015/145 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method, comprising:
introducing a reagent solution into a flow channel of a sensor that comprises:
a support frame comprising at least one cavity and at least one electrically conductive via;
at least one light detection device disposed within the at least one cavity that comprises a plurality of light sensors, device circuitry electrically coupled to the plurality of light sensors and the at least one electrically conductive via, and a detector surface that comprises reaction sites;
a support material disposed within the at least one cavity coupling the support frame and the at least one light detection device together; and
a lid extending over the at least one light detection device forming the flow channel between the lid and the detector surface; and
illuminating the detector surface with excitation light that passes through the lid.