US 11,747,261 B2
Hydrogel particles with tunable optical properties and methods for using the same
Jeffrey Kim, Berkeley, CA (US); Oliver Liu, San Francisco, CA (US); Jeremy Agresti, El Cerrito, CA (US); and Anh Tuan Nguyen, San Jose, CA (US)
Assigned to Slingshot Biosciences, Inc., Emeryville, CA (US)
Filed by Slingshot Biosciences, Inc., Emeryville, CA (US)
Filed on Jul. 20, 2020, as Appl. No. 16/933,028.
Application 16/933,028 is a continuation of application No. 15/625,394, filed on Jun. 16, 2017, granted, now 10,753,846.
Application 15/625,394 is a continuation of application No. 15/018,769, filed on Feb. 8, 2016, granted, now 9,714,897, issued on Jul. 25, 2017.
Claims priority of provisional application 62/184,192, filed on Jun. 24, 2015.
Claims priority of provisional application 62/114,004, filed on Feb. 9, 2015.
Prior Publication US 2020/0400546 A1, Dec. 24, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 15/10 (2006.01); G01N 15/14 (2006.01); C12Q 1/04 (2006.01); G01N 1/28 (2006.01); G01N 15/00 (2006.01)
CPC G01N 15/1012 (2013.01) [C12Q 1/04 (2013.01); G01N 1/28 (2013.01); G01N 15/1434 (2013.01); G01N 15/1459 (2013.01); G01N 2001/2893 (2013.01); G01N 2015/0065 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/1018 (2013.01); G01N 2015/149 (2013.01)] 41 Claims
OG exemplary drawing
 
1. A population of hydrogel particles, each hydrogel particle comprising:
(i) a polymer comprising a polymerized monomer and a co-monomer;
(ii) one or more biomolecules; and
(iii) a nanoparticle encapsulated within the hydrogel,
wherein the population hydrogel particles has an optical property that is substantially similar to a corresponding property of a target cell, the optical property provided by the co-monomer, a ratio of monomer to co-monomer, or the encapsulated nanoparticle.