	US 11,746,389 B2
	Method and kit of detecting the absence of micro-organisms
Matthew A. Crow, Oxfordshire (GB); Helen V. Bennett, Buckinghamshire (GB); Daniel S. Wratting, Oxfordshire (GB); and William H. Mullen, Berkshire (GB)
Assigned to Momentum Bioscience Limited, St. Mellons (GB)
Filed by Momentum Bioscience Limited, Wales (GB)
Filed on Jun. 20, 2022, as Appl. No. 17/844,498.
Application 17/844,498 is a continuation of application No. 17/002,559, filed on Aug. 25, 2020, abandoned.
Application 17/002,559 is a continuation of application No. 15/325,225, granted, now 10,793,917, issued on Oct. 6, 2020, previously published as PCT/GB2015/052006, filed on Jul. 10, 2015.
Claims priority of application No. 1412316 (GB), filed on Jul. 10, 2014.
Prior Publication US 2022/0372558 A1, Nov. 24, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/689 (2018.01); C12Q 1/6848 (2018.01); C12Q 1/04 (2006.01)

CPC C12Q 1/689 (2013.01) [C12Q 1/04 (2013.01); C12Q 1/6848 (2013.01); C12Q 2521/101 (2013.01); C12Q 2525/101 (2013.01); C12Q 2525/113 (2013.01); G01N 2333/91245 (2013.01)]

20 Claims

1. A method of detecting the presence of a micro-organism in a sample, the method comprising:

(a) contacting the sample with a nuclease resistant nucleic acid molecule comprising a plurality of nuclease resistant nucleotides which is either extended by polymerase activity or is ligated by ligase activity of the micro-organism in the sample,

(b) incubating the thus contacted sample under conditions suitable for polymerase activity or ligase activity; and

(c) detecting in the sample a nucleic acid molecule that has been extended by the polymerase activity or ligated by the ligase activity of the micro-organism as compared to a negative control, thereby indicating the presence of the micro-organism in the sample.