CPC C12Q 1/6811 (2013.01) [C12Q 1/48 (2013.01)] | 18 Claims |
1. A method of determining an association of a signal transduction pathway or receptor and an effector molecule of the signal transduction pathway or receptor, the method comprising: a) providing a eukaryotic cell comprising a recombinant molecule encoding a naturally occurring or functional variant of a eukaryotic signal transduction pathway or receptor and a polymerase, and further providing an effector molecule, wherein when the effector molecule interacts with the signal transduction pathway or receptor, transcription of the signal transduction pathway or receptor and polymerase takes place; b) isolating the eukaryotic cell; c) exposing the recombinant molecule to primers capable of amplifying the signal transduction pathway or receptor and polymerase under conditions sufficient for amplification of the recombinant molecule; and d); detecting interaction between the signal transduction pathway or receptor and an effector molecule by detecting that amplification occurred.
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