US 11,746,374 B2
Methods and platform for screening and selecting metabolites and their receptors
Andrew Ellington, Austin, TX (US); Jimmy Gollihar, Hewitt, TX (US); Katy Kao, College Station, TX (US); and Elizabeth Gardner, Austin, TX (US)
Assigned to BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM, Austin, TX (US); and THE TEXAS A&M UNIVERSITY, College Station, TX (US)
Appl. No. 16/622,445
Filed by BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM, Austin, TX (US); and THE TEXAS A&M UNIVERSITY SYSTEM, College Station, TX (US)
PCT Filed Jun. 15, 2018, PCT No. PCT/US2018/037818
§ 371(c)(1), (2) Date Dec. 13, 2019,
PCT Pub. No. WO2018/232282, PCT Pub. Date Dec. 20, 2018.
Claims priority of provisional application 62/520,189, filed on Jun. 15, 2017.
Prior Publication US 2020/0208201 A1, Jul. 2, 2020
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6811 (2018.01); C12Q 1/48 (2006.01)
CPC C12Q 1/6811 (2013.01) [C12Q 1/48 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method of determining an association of a signal transduction pathway or receptor and an effector molecule of the signal transduction pathway or receptor, the method comprising: a) providing a eukaryotic cell comprising a recombinant molecule encoding a naturally occurring or functional variant of a eukaryotic signal transduction pathway or receptor and a polymerase, and further providing an effector molecule, wherein when the effector molecule interacts with the signal transduction pathway or receptor, transcription of the signal transduction pathway or receptor and polymerase takes place; b) isolating the eukaryotic cell; c) exposing the recombinant molecule to primers capable of amplifying the signal transduction pathway or receptor and polymerase under conditions sufficient for amplification of the recombinant molecule; and d); detecting interaction between the signal transduction pathway or receptor and an effector molecule by detecting that amplification occurred.