US 11,746,361 B2
Metabolic engineering for simultaneous consumption of Xylose and glucose for production of chemicals from second generation sugars
Veronica Maria Rodege Gogola Kolling, Camacari (BR); Ane Fernanda Beraldi Zeidler, Camacari (BR); and Lucas Pedersen Parizzi, Camacari (BR)
Assigned to BRASKEM S.A., Camaçari (BR)
Filed by Braskem S.A., Camacari (BR)
Filed on Apr. 6, 2020, as Appl. No. 16/841,312.
Claims priority of provisional application 62/829,398, filed on Apr. 4, 2019.
Prior Publication US 2020/0318146 A1, Oct. 8, 2020
Int. Cl. C12P 7/18 (2006.01); C12N 1/20 (2006.01); C12P 7/42 (2006.01); C12N 9/88 (2006.01); C12N 9/10 (2006.01); C12N 9/12 (2006.01); C12N 9/04 (2006.01); C12N 9/90 (2006.01); C12P 7/28 (2006.01); C12N 9/92 (2006.01); C12P 7/04 (2006.01); C12N 1/21 (2006.01)
CPC C12P 7/18 (2013.01) [C12N 1/20 (2013.01); C12N 9/0006 (2013.01); C12N 9/1029 (2013.01); C12N 9/1205 (2013.01); C12N 9/13 (2013.01); C12N 9/88 (2013.01); C12N 9/90 (2013.01); C12N 9/92 (2013.01); C12P 7/04 (2013.01); C12P 7/28 (2013.01); C12P 7/42 (2013.01); C12P 2203/00 (2013.01); C12Y 503/01005 (2013.01); Y02E 50/10 (2013.01)] 20 Claims
 
1. A recombinant microorganism capable of producing a fermentation product from a feedstock comprising xylose and glucose, wherein the recombinant microorganism simultaneously utilizes the xylose and the glucose, and wherein the recombinant microorganism comprises:
(a) a deletion or an inactivation of nucleic acid sequences that encode a xylose ABC transporter and an arabinose ABC transporter;
(b) one or more endogenous or exogenous nucleic acid sequences that encodes a C5 sugar symporter operatively linked to one or more constitutive promoters, wherein the C5 sugar symporter comprises: (1) a xylose symporter and/or a (2) an arabinose symporter;
(c) one or more endogenous or exogenous nucleic acid sequences that encodes (1) a xylose isomerase operatively linked to one or more constitutive promoters, and a deletion or an inactivation of one or more nucleic acid sequences that encodes a xylulokinase or (2) a xylose dehydrogenase operatively linked to one or more constitutive promoters and a deletion or an inactivation of one or more nucleic acid sequences that encodes a xylose isomerase and/or a xylulokinase;
(d) a deletion or an inactivation of one or more nucleic acid sequences that encodes a glycolate dehydrogenase; and
(e) a native functional phosphotransferase system (PTS) and a native cAMP receptor protein (CRP);
wherein the recombinant microorganism is E. coli.