CPC C12P 7/18 (2013.01) [C12N 1/20 (2013.01); C12N 9/0006 (2013.01); C12N 9/1029 (2013.01); C12N 9/1205 (2013.01); C12N 9/13 (2013.01); C12N 9/88 (2013.01); C12N 9/90 (2013.01); C12N 9/92 (2013.01); C12P 7/04 (2013.01); C12P 7/28 (2013.01); C12P 7/42 (2013.01); C12P 2203/00 (2013.01); C12Y 503/01005 (2013.01); Y02E 50/10 (2013.01)] | 20 Claims |
1. A recombinant microorganism capable of producing a fermentation product from a feedstock comprising xylose and glucose, wherein the recombinant microorganism simultaneously utilizes the xylose and the glucose, and wherein the recombinant microorganism comprises:
(a) a deletion or an inactivation of nucleic acid sequences that encode a xylose ABC transporter and an arabinose ABC transporter;
(b) one or more endogenous or exogenous nucleic acid sequences that encodes a C5 sugar symporter operatively linked to one or more constitutive promoters, wherein the C5 sugar symporter comprises: (1) a xylose symporter and/or a (2) an arabinose symporter;
(c) one or more endogenous or exogenous nucleic acid sequences that encodes (1) a xylose isomerase operatively linked to one or more constitutive promoters, and a deletion or an inactivation of one or more nucleic acid sequences that encodes a xylulokinase or (2) a xylose dehydrogenase operatively linked to one or more constitutive promoters and a deletion or an inactivation of one or more nucleic acid sequences that encodes a xylose isomerase and/or a xylulokinase;
(d) a deletion or an inactivation of one or more nucleic acid sequences that encodes a glycolate dehydrogenase; and
(e) a native functional phosphotransferase system (PTS) and a native cAMP receptor protein (CRP);
wherein the recombinant microorganism is E. coli.
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