US 11,746,360 B2
Eukaryotic cell transfection systems and related methods
Florian M. Wurm, Montreux (CH); Maria J. Wurm, Montreux (CH); Maria de Lourdes Rodrigues, Collombey (CH); Divor Kiseljak, Renens (CH); Cedric Bürki, Pully (CH); Cyril Pugin, Vevey (CH); Guillaume Raussin, Aigle (CH); and Julie Heymoz, Lausanne (CH)
Filed by Florian M. Wurm, Montreux (CH); Maria J. Wurm, Montreux (CH); Maria de Lourdes Rodrigues, Collombey (CH); Divor Kiseljak, Renens (CH); Cedric Bürki, Pully (CH); Cyril Pugin, Vevey (CH); Guillaume Raussin, Aigle (CH); and Julie Heymoz, Lausanne (CH)
Filed on Feb. 11, 2020, as Appl. No. 16/788,303.
Claims priority of provisional application 62/803,639, filed on Feb. 11, 2019.
Prior Publication US 2020/0263203 A1, Aug. 20, 2020
Int. Cl. C12N 15/87 (2006.01); C12N 5/07 (2010.01); C12N 5/071 (2010.01)
CPC C12N 15/87 (2013.01) [C12N 5/0601 (2013.01); C12N 5/0602 (2013.01); C12N 2500/50 (2013.01); C12N 2510/04 (2013.01)] 18 Claims
 
1. A highly-efficient eukaryotic cell transfection (HECT) system that is capable of mediating transfection of purified, unencumbered exogenous nucleic acid into a eukaryotic cell comprising:
a cell transfection medium pre-conditioned with a positively charged polymer that is not associated with nucleic acid;
a eukaryotic cell for combination with said cell transfection medium to produce cell-supplemented pre-conditioned cell transfection medium; and
a purified, unencumbered exogenous nucleic acid for combination with the cell-supplemented pre-conditioned cell transfection medium, wherein said purified, unencumbered exogenous nucleic acid is free from the components selected from the group consisting of proteins, cationic lipids, cationic polymers, liposome forming components, calcium phosphates, calcium chloride, nano-particles, metals, polymeric gene carriers, dendrimers, and cyclodextrins, for transfection of said purified, unencumbered exogenous nucleic acid into said eukaryotic cell.