	US 11,746,341 B2
	Performance-enhanced and storage stable protease variants
Christian Degering, Erkrath (DE); Layla Fernandez, Cologne (DE); Sabine Griemert, Monheim am Rhein (DE); Nina Mussmann, Willich (DE); Inken Prueser, Duesseldorf (DE); Daria Strauss, Duesseldorf (DE); and Susanne Wieland, Dormagen/Zons (DE)
Assigned to Henkel AG & Co. KGaA, Duesseldorf (DE)
Filed by Henkel AG & Co. KGaA, Duesseldorf (DE)
Filed on Nov. 28, 2019, as Appl. No. 16/699,013.
Claims priority of application No. 18209177 (EP), filed on Nov. 29, 2018.
Prior Publication US 2020/0172890 A1, Jun. 4, 2020
Int. Cl. C12N 9/54 (2006.01)

CPC C12N 9/54 (2013.01) [C12Y 304/21062 (2013.01)]

12 Claims

1. A protease comprising an amino acid sequence having at least 70% sequence identity to SEQ ID NO:1, wherein the protease comprises: i) first amino acid substitutions at at least four positions corresponding to positions 3, 4, 99, and 199; wherein the amino acid substitutions are S3T, V4I, R99E, and V199T; and ii) one or more second amino acid substitutions at positions corresponding to positions A267S+N255Y; N198D+S234A+L21F; N212D; G205D; S182C; R44S+S234A; 253C; I105V; N177D; S9C; N198D; S259C; I159L+Q230E; P204L; L21F; S139R+S250N; N42S; V137I; V145I; S206A; G113A+L21W; A112V; A168V; Q176E+A131D; or T1418+S210C.