CPC C12N 7/045 (2013.01) [C07K 14/005 (2013.01); C07K 14/1825 (2013.01); C12N 7/00 (2013.01); A61K 2039/5254 (2013.01); C12N 2770/24122 (2013.01); C12N 2770/24162 (2013.01); Y02A 50/30 (2018.01)] | 4 Claims |
1. A tetravalent immunogenic composition comprising nucleic acids encoding each of DEN1, DEN2, DEN3, and DEN4, wherein each nucleic acid has a Δ30/Δ31 mutation in the 3′-untranslated region (3′-UTR), wherein:
the mutation deletes nucleotides 174-145 and nucleotides 258-228 of DEN1;
the mutation deletes nucleotides 173-144 and nucleotides 258-228 of DEN2;
the mutation deletes nucleotides 173-143 and nucleotides 258-228 of DEN3; and
the mutation deletes nucleotides 172-143 and nucleotides 258-228 of DEN4, designated with the reverse order numbering system.
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