CPC C12N 5/0697 (2013.01) [B29C 64/118 (2017.08); B33Y 70/00 (2014.12); B33Y 80/00 (2014.12); C12M 21/08 (2013.01); C12M 25/14 (2013.01); C12M 33/00 (2013.01); C12N 5/0658 (2013.01); G01N 33/5061 (2013.01); B33Y 10/00 (2014.12); C12N 2533/50 (2013.01)] | 13 Claims |
1. A method for evaluating the effect of a test agent and/or condition on the contractile function of a mammalian muscle cell, comprising the steps of a) contacting a synthetic living tissue structure comprising a continuous fiber of mammalian muscle cells dispensed from a bioprinter in overlapping layers with the test agent and/or condition, wherein said fiber comprises a solidified biocompatible matrix comprising said cells and said solidified biocompatible matrix is homogeneous in radial cross-section throughout said fiber and is capable of maintaining the structural integrity and shape fidelity of said fiber upon deposition and without any additional cross-linking steps following deposition, and wherein the structural integrity of the fiber is maintained between each layer and the layers are structurally discrete; and b) determining the effect of said agent and/or condition on said mammalian muscle cells by measuring a dimensional change in the structure.
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