US 11,746,333 B2
Continuously bioprinted multilayer tissue structure
Simon Beyer, Richmond (CA); Tamer Mohamed, Richmond (CA); Sheng Pan, Vancouver (CA); and Sam Wadsworth, Vancouver (CA)
Assigned to ASPECT BIOSYSTEMS LTD.
Appl. No. 15/736,743
Filed by ASPECT BIOSYSTEMS LTD., Vancouver (CA)
PCT Filed Jun. 16, 2016, PCT No. PCT/CA2016/050707
§ 371(c)(1), (2) Date Dec. 14, 2017,
PCT Pub. No. WO2016/201577, PCT Pub. Date Dec. 22, 2016.
Claims priority of provisional application 62/305,422, filed on Mar. 8, 2016.
Claims priority of provisional application 62/180,174, filed on Jun. 16, 2015.
Prior Publication US 2018/0171304 A1, Jun. 21, 2018
Int. Cl. C12N 5/071 (2010.01); C12N 5/077 (2010.01); C12M 1/12 (2006.01); G01N 33/50 (2006.01); B33Y 80/00 (2015.01); B33Y 70/00 (2020.01); C12M 1/26 (2006.01); C12M 3/00 (2006.01); B29C 64/118 (2017.01); B33Y 10/00 (2015.01)
CPC C12N 5/0697 (2013.01) [B29C 64/118 (2017.08); B33Y 70/00 (2014.12); B33Y 80/00 (2014.12); C12M 21/08 (2013.01); C12M 25/14 (2013.01); C12M 33/00 (2013.01); C12N 5/0658 (2013.01); G01N 33/5061 (2013.01); B33Y 10/00 (2014.12); C12N 2533/50 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A method for evaluating the effect of a test agent and/or condition on the contractile function of a mammalian muscle cell, comprising the steps of a) contacting a synthetic living tissue structure comprising a continuous fiber of mammalian muscle cells dispensed from a bioprinter in overlapping layers with the test agent and/or condition, wherein said fiber comprises a solidified biocompatible matrix comprising said cells and said solidified biocompatible matrix is homogeneous in radial cross-section throughout said fiber and is capable of maintaining the structural integrity and shape fidelity of said fiber upon deposition and without any additional cross-linking steps following deposition, and wherein the structural integrity of the fiber is maintained between each layer and the layers are structurally discrete; and b) determining the effect of said agent and/or condition on said mammalian muscle cells by measuring a dimensional change in the structure.