US 11,746,327 B2
Composition for culturing NK cells and method for culturing NK cells using same
Hee Jung An, Seoul (KR); Yeon Ho Choi, Seoul (KR); Eun Jin Lim, Seongnam-si (KR); Yong Wha Moon, Seongnam-si (KR); and Se Wha Kim, Seongnam-si (KR)
Assigned to SUNGKWANG MEDICAL FOUNDATION, Seoul (KR)
Appl. No. 16/645,215
Filed by SUNGKWANG MEDICAL FOUNDATION, Seoul (KR)
PCT Filed Nov. 20, 2018, PCT No. PCT/KR2018/014294
§ 371(c)(1), (2) Date Mar. 6, 2020,
PCT Pub. No. WO2019/103436, PCT Pub. Date May 31, 2019.
Claims priority of application No. 10-2017-0158577 (KR), filed on Nov. 24, 2017.
Prior Publication US 2021/0095249 A1, Apr. 1, 2021
Int. Cl. C12N 5/0783 (2010.01); A61P 35/00 (2006.01); A61K 35/17 (2015.01)
CPC C12N 5/0646 (2013.01) [A61K 35/17 (2013.01); A61P 35/00 (2018.01)] 2 Claims
 
1. A method of culturing natural killer cells (NK cells), the method comprising culturing the NK cells in a composition for culturing NK cells, the composition comprising IL-15 in an amount of 5.0 ng/ml to 20 ng/ml, IL-18 in an amount of 0.25 ng/ml to 2500 ng/ml, IL-27 in an amount of 0.20 ng/ml to 2000 ng/ml, and insulin transferrin selenium (ITS).