CPC C12N 5/0634 (2013.01) [A01N 1/0284 (2013.01); A61K 35/51 (2013.01); A61P 7/00 (2018.01); A61P 31/00 (2018.01); A61P 35/02 (2018.01)] | 25 Claims |
1. A method of treating a hematological disease in a subject in need thereof, the method comprising:
(a) separating a single umbilical cord blood unit suitable for transplantation into said subject into (i) a first, selected blood cell fraction comprising CD133+/CD34+ selected cells and (ii) a second, unselected blood cell fraction comprising CD133/CD34 negative cells;
(b) ex vivo culturing said first blood cell fraction comprising CD133+/CD34+ selected cells under conditions allowing for cell proliferation, said conditions comprising providing nutrients, serum and a combination of cytokines including each of stem cell factor, thrombopoietin, FLt3 ligand and IL-6 and nicotinamide in an amount between 1.0 mM to 10 mM;
(c) cryopreserving said ex-vivo cultured first, CD133+/CD34+ selected blood cell fraction and said uncultured second, unselected blood cell fraction from step (b),
(d) thawing said ex-vivo cultured first selected and said uncultured second, unselected blood cell fractions, and
(e) transplanting the thawed ex-vivo cultured first selected and uncultured second unselected blood cell fractions from step (d) into a subject in need thereof,
thereby treating said hematological disease in said subject.
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