	US 11,746,324 B2
	Large scale production of retinal pigment epithelial cells
Benjamin Eithan Reubinoff, Doar-Na HaEla (IL); and Orna Singer, Jerusalem (IL)
Assigned to Hadasit Medical Research Services and Development Ltd., Jerusalem (IL)
Filed by Hadasit Medical Research Services and Development Ltd., Jerusalem (IL)
Filed on Dec. 10, 2021, as Appl. No. 17/547,863.
Application 17/547,863 is a continuation of application No. 15/748,541, granted, now 11,230,696, previously published as PCT/IL2016/050829, filed on Jul. 28, 2016.
Application 15/748,541 is a continuation of application No. 62/198,160, filed on Jul. 29, 2015.
Prior Publication US 2022/0098551 A1, Mar. 31, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 5/079 (2010.01); A61P 27/02 (2006.01); A61K 9/00 (2006.01); A61K 35/30 (2015.01)

CPC C12N 5/0621 (2013.01) [A61K 9/0051 (2013.01); A61K 35/30 (2013.01); A61P 27/02 (2018.01); C12N 2500/38 (2013.01); C12N 2501/115 (2013.01); C12N 2501/15 (2013.01); C12N 2502/13 (2013.01); C12N 2506/02 (2013.01); C12N 2533/52 (2013.01); C12N 2533/54 (2013.01)]

25 Claims

1. A method of production of retinal pigment epithelial (RPE) cells comprising:

(a) culturing human pluripotent stem cells in a human feeder cell-conditioned medium in the absence of feeder cells to obtain a cultured population of human pluripotent stem cells;

(b) differentiating said cultured population of human pluripotent stem cells in a medium comprising a differentiating agent to obtain differentiating cells; and

(c) further differentiating said differentiating cells in a medium comprising one or more members of the TGFβ superfamily, thereby generating the RPE cells; wherein a yield of between about 50×10⁶to about 150×10⁶RPE cells per 175 mL container is capable of being or can be obtained.