CPC C12N 5/0603 (2013.01) [C12N 5/0606 (2013.01); C12N 2501/115 (2013.01); C12N 2501/119 (2013.01); C12N 2501/15 (2013.01); C12N 2501/155 (2013.01); C12N 2501/16 (2013.01); C12N 2501/385 (2013.01); C12N 2501/415 (2013.01); C12N 2502/13 (2013.01); C12N 2506/02 (2013.01)] | 8 Claims |
1. An in vitro cell culture comprising:
human pancreatic and duodenal homeobox 1 (PDX1)-negative definitive endoderm cells;
a medium comprising an effective amount of B27, a retinoid and a fibroblast growth factor (FGF), to differentiate the PDX1-negative definitive endoderm cells to PDX1-positive foregut endoderm cells; and
PDX1-positive foregut endoderm cells,
such that the amount of PDX1 expressed by the PDX1-positive foregut endoderm cells is increased in the in vitro cell culture as compared to a second in vitro cell culture comprising human PDX1-negative definitive endoderm cells, PDX1-positive foregut endoderm cells, and the medium without B27.
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