CPC C12N 1/205 (2021.05) [C12N 9/1029 (2013.01); C12N 15/74 (2013.01); C12R 2001/63 (2021.05)] | 18 Claims |
1. A method of cloning a nucleic acid or of producing a protein comprising:
culturing a recombinant Vibrio sp. organism comprising:
an exogenous nucleic acid sequence for cloning by the organism, or an exogenous nucleic acid sequence encoding a heterologous protein or peptide for production by the organism;
a genetic modification selected from deletion, inactivation, or disruption of the lpxL gene or the lpxM gene, wherein the organism produces substantially less endotoxin compared to a corresponding organism not comprising the deletion, inactivation, or disruption and cultivated under the same conditions; and
wherein the recombinant Vibrio sp. organism exhibits a growth rate of at least 60% of the growth rate of the corresponding organism when cultivated under the same conditions;
wherein the organism has an endotoxin level of less than 50 EU/ml of purified lipopolysaccharide; and
harvesting the nucleic acid sequence cloned by the organism, or the protein or peptide produced by the organism.
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