US 11,746,316 B2
Three-dimensional structuring method and three-dimensional structuring system of cells
Yoshiyuki Sankai, Ibaraki (JP)
Assigned to CYBERDYNE Inc., Tsukuba (JP); and University of Tsukuba, Tsukuba (JP)
Appl. No. 16/324,496
Filed by CYBERDYNE Inc., Tsukuba (JP); and University of Tsukuba, Tsukuba (JP)
PCT Filed Jun. 9, 2017, PCT No. PCT/JP2017/021552
§ 371(c)(1), (2) Date Feb. 8, 2019,
PCT Pub. No. WO2018/029970, PCT Pub. Date Feb. 15, 2018.
Claims priority of application No. 2016-158063 (JP), filed on Aug. 10, 2016.
Prior Publication US 2019/0169558 A1, Jun. 6, 2019
Int. Cl. C12M 1/12 (2006.01); C12M 1/34 (2006.01); C12M 1/36 (2006.01); C12M 3/06 (2006.01); C12N 5/077 (2010.01); C12M 3/00 (2006.01); C12N 5/00 (2006.01); C12M 1/00 (2006.01)
CPC C12M 25/12 (2013.01) [C12M 3/00 (2013.01); C12M 21/08 (2013.01); C12M 25/02 (2013.01); C12M 27/16 (2013.01); C12M 29/10 (2013.01); C12M 29/16 (2013.01); C12M 41/14 (2013.01); C12M 41/34 (2013.01); C12M 41/44 (2013.01); C12M 41/48 (2013.01); C12N 5/00 (2013.01); C12N 5/0658 (2013.01)] 5 Claims
OG exemplary drawing
 
1. A three-dimensional culture method of cells, comprising:
positioning a plurality of fibers in a flow path spatial part, the flow path includes a space surrounded by an inner wall surface from a supply port to a discharge port of a culture solution, so that a longitudinal direction of each of the fibers is along a direction of the flow path while a predetermined interval is mutually maintained between the fibers;
continuously supplying a culture solution to the flow path through the supply port;
placing a plurality of cell clusters in the flow path at predetermined timing, and causing the cell clusters to run with a liquid flow of the culture solution being supplied in the flow path from the supply port;
stacking the cell clusters on an outer surface of each of the plurality of fibers;
culturing each of the cell clusters;
controlling a flow rate of the culture solution being supplied into the flow path;
adjusting a number of the cell clusters being placed in the flow path; and
adjusting a placement timing of the cell clusters into the flow path,
wherein a flat plate is provided in a downstream section of the flow path spatial part for serving as a growth support for the cell clusters being cultured in the flow path.