CPC C12M 25/12 (2013.01) [C12M 3/00 (2013.01); C12M 21/08 (2013.01); C12M 25/02 (2013.01); C12M 27/16 (2013.01); C12M 29/10 (2013.01); C12M 29/16 (2013.01); C12M 41/14 (2013.01); C12M 41/34 (2013.01); C12M 41/44 (2013.01); C12M 41/48 (2013.01); C12N 5/00 (2013.01); C12N 5/0658 (2013.01)] | 5 Claims |
1. A three-dimensional culture method of cells, comprising:
positioning a plurality of fibers in a flow path spatial part, the flow path includes a space surrounded by an inner wall surface from a supply port to a discharge port of a culture solution, so that a longitudinal direction of each of the fibers is along a direction of the flow path while a predetermined interval is mutually maintained between the fibers;
continuously supplying a culture solution to the flow path through the supply port;
placing a plurality of cell clusters in the flow path at predetermined timing, and causing the cell clusters to run with a liquid flow of the culture solution being supplied in the flow path from the supply port;
stacking the cell clusters on an outer surface of each of the plurality of fibers;
culturing each of the cell clusters;
controlling a flow rate of the culture solution being supplied into the flow path;
adjusting a number of the cell clusters being placed in the flow path; and
adjusting a placement timing of the cell clusters into the flow path,
wherein a flat plate is provided in a downstream section of the flow path spatial part for serving as a growth support for the cell clusters being cultured in the flow path.
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