	US 11,746,130 B2
	Polypeptide and method of producing IMP using the same
Jin Ah Rho, Suwon-si (KR); Byoung Hoon Yoon, Seoul (KR); So-jung Park, Suwon-si (KR); Min Ji Baek, Suwon-si (KR); and Ji Hye Lee, Anyang-si (KR)
Assigned to CJ CHEILJEDANG CORPORATION, Seoul (KR)
Filed by CJ CHEILJEDANG CORPORATION, Seoul (KR)
Filed on Jun. 6, 2022, as Appl. No. 17/833,539.
Application 17/833,539 is a division of application No. 16/346,725, granted, now 11,384,124, previously published as PCT/KR2019/000168, filed on Jan. 4, 2019.
Claims priority of application No. 10-2018-0001399 (KR), filed on Jan. 4, 2018.
Prior Publication US 2022/0348616 A1, Nov. 3, 2022
Int. Cl. C07K 14/34 (2006.01); C12N 1/20 (2006.01); C12P 19/32 (2006.01); C12R 1/15 (2006.01)

CPC C07K 14/34 (2013.01) [C12N 1/20 (2013.01); C12N 1/205 (2021.05); C12P 19/32 (2013.01); C12R 2001/15 (2021.05)]

8 Claims

1. A polynucleotide encoding a protein variant having activity of exporting 5′-inosine monophosphate,

wherein the protein variant has at least 90% sequence identity to SEQ ID NO:2, and comprises one or more substitutions selected from the group consisting of: a substitution of the 123rd amino acid with cysteine, a substitution of the 243rd amino acid with valine, a substitution of the 387th amino acid with threonine, a substitution of the 405th amino acid with tyrosine, a substitution of the 413th amino acid with threonine, and a substitution of the 458th amino acid with lysine, and combinations thereof, in the amino acid sequence of SEQ ID NO: 2.