US 11,745,412 B2
Cross-linkable microgel composite matrix bath for embedded bioprinting of perfusable tissue constructs
Ashley M. Compaan, Gainesville, FL (US); Yong Huang, Gainesville, FL (US); Kaidong Song, Gainesville, FL (US); and Wenxuan Chai, Gainesville, FL (US)
Assigned to UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATED, Gainesville, FL (US)
Filed by University of Florida Research Foundation, Incorporated, Gainesville, FL (US)
Filed on Jul. 7, 2022, as Appl. No. 17/859,593.
Application 17/859,593 is a division of application No. 16/703,691, filed on Dec. 4, 2019, granted, now 11,413,808.
Claims priority of provisional application 62/778,639, filed on Dec. 12, 2018.
Prior Publication US 2022/0410470 A1, Dec. 29, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. B29C 64/118 (2017.01); B33Y 70/10 (2020.01); B29C 64/40 (2017.01); A61L 27/44 (2006.01); A61L 27/56 (2006.01); A61L 27/52 (2006.01); B29K 105/00 (2006.01); B29K 105/16 (2006.01); B29L 31/40 (2006.01)
CPC B29C 64/118 (2017.08) [A61L 27/44 (2013.01); A61L 27/52 (2013.01); A61L 27/56 (2013.01); B29C 64/40 (2017.08); B33Y 70/10 (2020.01); B29K 2089/00 (2013.01); B29K 2105/0061 (2013.01); B29K 2105/16 (2013.01); B29L 2031/40 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for embedded printing of perfusable tissue constructs, the method comprising:
providing a composite matrix bath comprising a microgel filler and a hydrogel precursor, the microgel filler comprising one or more of gellan or gelatin, the hydrogel precursor comprising gelatin;
disposing a volume of a sacrificial material into the composite matrix bath; and
allowing the volume of the sacrificial material to at least partially solidify.