US 11,745,183 B2
Microtiter plate and uses thereof
Jose Jimenez, Madison, WI (US); Brian Johnson, Madison, WI (US); and David Beebe, Monona, WI (US)
Assigned to ONEXIO BIOSYSTEMS LLC, Madison, WI (US)
Filed by ONEXIO BIOSYSTEMS LLC, Madison, WI (US)
Filed on Nov. 25, 2019, as Appl. No. 16/694,096.
Application 16/694,096 is a continuation of application No. 16/168,476, filed on Oct. 23, 2018, granted, now 10,518,266.
Application 16/168,476 is a continuation of application No. PCT/US2017/041204, filed on Jul. 7, 2017.
Claims priority of provisional application 62/359,977, filed on Jul. 8, 2016.
Claims priority of provisional application 62/359,483, filed on Jul. 7, 2016.
Prior Publication US 2020/0086311 A1, Mar. 19, 2020
Int. Cl. B01L 3/00 (2006.01); C12M 1/32 (2006.01); C12M 1/00 (2006.01); C12M 1/18 (2006.01); C12M 3/06 (2006.01); C12M 1/04 (2006.01); C12M 1/12 (2006.01)
CPC B01L 3/5085 (2013.01) [C12M 1/18 (2013.01); C12M 23/12 (2013.01); C12M 23/16 (2013.01); C12M 23/24 (2013.01); C12M 23/34 (2013.01); C12M 25/04 (2013.01); C12M 29/04 (2013.01); B01L 2300/069 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/0851 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A cell culture device, comprising:
i) a plurality of first wells having a sidewall, an end wall, and a bottom wall, wherein a distance from said bottom wall of said first wells and an upper edge of said sidewall of said first wells defining a depth of said first wells;
ii) a plurality of second wells, said plurality of second wells having a side wall, an end wall, and a bottom wall, wherein a distance from said bottom wall of said second wells and an upper edge of said sidewall of said second wells defining a depth of said second wells;
wherein said second wells are adjacent with said first wells such that said end wall of said first wells is said end wall of said second wells;
wherein said end wall connects with said bottom walls of said first and second wells to form a physical and fluidic barrier between said first and second wells;
wherein at least a portion of said end wall has a height less than said first and second depths, such that fluid in either first or second wells flows over said end wall when said fluid has a level exceeding said height and wherein fluid is prevented from moving between said first and second wells when said fluid has a level less than said height.