US 11,745,177 B2
Ultrasound lysing of whole blood
Brian Joseph Bosy, Hull, MA (US); and Josef Kerimo, Concord, MA (US)
Assigned to INSTRUMENTATION LABORATORY COMPANY, Bedford, MA (US)
Filed by Instrumentation Laboratory Company, Bedford, MA (US)
Filed on Jan. 9, 2020, as Appl. No. 16/738,915.
Claims priority of provisional application 62/790,520, filed on Jan. 10, 2019.
Prior Publication US 2020/0222894 A1, Jul. 16, 2020
Int. Cl. B01L 3/00 (2006.01); G01N 1/28 (2006.01); G01N 21/31 (2006.01); G01N 33/72 (2006.01)
CPC B01L 3/502 (2013.01) [G01N 1/28 (2013.01); G01N 21/31 (2013.01); G01N 33/721 (2013.01); B01L 2200/04 (2013.01); B01L 2300/06 (2013.01); B01L 2300/08 (2013.01); B01L 2300/12 (2013.01)] 20 Claims
OG exemplary drawing
 
1. An apparatus comprising:
a cartridge configured for removable installation in a blood analyzing instrument, the cartridge comprising:
a lysing chamber configured to receive a blood sample, the lysing chamber comprising a wall, the wall comprising a first part and a second part that surrounds the first part, the first part being thicker than the second part, and the first part comprising at least one interface surface configured to contact an ultrasonic transducer of the blood analyzing instrument and to transmit ultrasonic energy from the ultrasonic transducer to the blood sample to lyse the blood sample and thereby produce a lysed blood sample; and
a measurement chamber separate from the lysing chamber, the measurement chamber being configured to receive the lysed blood sample from the lysing chamber, and the measurement chamber being configured to enable performing a CO-Ox (CO-oximetry) measurement of the lysed blood sample by the blood analyzing instrument;
wherein the lysing chamber has a tear-shaped geometry, the tear-shaped geometry gradually expanding toward a substantially circular region, the first part being over the substantially circular region and the ultrasonic energy being transmitted to lyse the blood sample in the substantially circular region.