US 11,744,905 B2
Skin barrier preparation and method therefor
Jalil Bensaci, Issy-les-Moulineaux (FR); Thierry Oddos, Clamart (FR); and Georgios N. Stamatas, Issy-les-Moulineaux (FR)
Assigned to Johnson & Johnson Consumer Inc., Skillman, NJ (US)
Filed by Johnson & Johnson Consumer Inc., Skillman, NJ (US)
Filed on Dec. 14, 2018, as Appl. No. 16/220,065.
Claims priority of provisional application 62/613,878, filed on Jan. 5, 2018.
Prior Publication US 2019/0212324 A1, Jul. 11, 2019
Int. Cl. A61K 49/00 (2006.01); G01N 33/50 (2006.01); A61K 8/49 (2006.01); A61Q 17/00 (2006.01); G06F 30/20 (2020.01); A61K 9/70 (2006.01); G16H 50/50 (2018.01); G06F 16/245 (2019.01); G01J 3/44 (2006.01); G01J 3/28 (2006.01)
CPC A61K 49/0006 (2013.01) [A61K 8/4953 (2013.01); A61K 9/7038 (2013.01); A61K 9/7084 (2013.01); A61K 9/7092 (2013.01); A61Q 17/00 (2013.01); G01J 3/44 (2013.01); G01N 33/5008 (2013.01); G01N 33/5088 (2013.01); G06F 16/245 (2019.01); G06F 30/20 (2020.01); G16H 50/50 (2018.01); G01J 2003/2866 (2013.01)] 6 Claims
 
1. A non-invasive in vivo method of evaluating a potential impact of a barrier system on infant skin, comprising:
a) topically applying said barrier system in a non-invasive in vivo method to adult skin;
b) topically applying a marker to said barrier system treated adult skin;
c) applying confocal analysis to measure-penetration of said marker into said barrier system treated adult skin;
d) using a computational model of adult skin penetration to visualize penetration of the marker by optimizing penetration parameters so that the model of adult skin penetration profiles match experimental data;
e) transferring the optimized penetration parameters to a computational model of infant skin; and
f) determining the penetration of the marker in the computational model of infant skin;
wherein step c) comprises following a concentration profile of said marker through the skin, wherein said concentration profile is measured using confocal Raman micro-spectroscopy (CRM) or confocal fluorescence microscopy;
wherein said penetration parameters are selected from the group consisting of skin surface concentration (Csurface), permeability coefficient for the stratum corneum (PSC) and permeability coefficient for the viable epidermis (PVE); and
wherein said penetration parameters are transferred in step e) such that the skin surface concentration (Csurface) parameter is higher in the computational model of infant skin than in the computational model of adult skin penetration, and such that the permeability coefficient for the stratum corneum (PSC) and the permeability coefficient for the viable epidermis (PVE) are the same between the two models.