US 11,744,886 B2
Method for producing RNA compositions
Thorsten Mutzke, Reutlingen (DE); Markus Kreuz, Kohlberg (DE); Stefanie Sewing, Tübingen (DE); Fabian Johannes Eber, Stuttgart (DE); Wenke Wagner, Reutlingen (DE); Michael Sonntag, Tübingen (DE); Michael Wiggenhorn, Munich (DE); and Katharina Kolland, Augsburg (DE)
Assigned to CureVac Manufacturing GmbH, Tübingen (DE)
Filed by CureVac Manufacturing GmbH, Tübingen (DE)
Filed on Apr. 2, 2020, as Appl. No. 16/838,746.
Application 16/838,746 is a continuation of application No. 15/566,010, granted, now 10,653,768, previously published as PCT/EP2016/000607, filed on Apr. 13, 2016.
Claims priority of application No. PCT/EP2015/000771 (WO), filed on Apr. 13, 2015.
Prior Publication US 2020/0246451 A1, Aug. 6, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 39/145 (2006.01); A61K 48/00 (2006.01); A61K 47/64 (2017.01); A61K 9/51 (2006.01); A61K 39/12 (2006.01); A61K 39/00 (2006.01)
CPC A61K 39/145 (2013.01) [A61K 9/5169 (2013.01); A61K 9/5192 (2013.01); A61K 39/12 (2013.01); A61K 47/646 (2017.08); A61K 47/6455 (2017.08); A61K 2039/55555 (2013.01); C12N 2760/16134 (2013.01)] 35 Claims
 
1. A method for producing a liquid composition comprising a nanoparticle comprising at least one long-chain RNA and at least one cationic or polycationic compound,
wherein the method comprises the steps of:
(a) providing a first liquid composition comprising the at least one long-chain RNA that comprises from 200 to 15,000 nucleotides,
(b) providing a second liquid composition comprising the at least one cationic or polycationic compound,
(c) introducing the first liquid composition and the second liquid composition into at least one reactor, wherein the first liquid composition and/or the second liquid composition are introduced at a flow rate of at least 5 mL/minute, and
(d) recovering the product liquid composition comprising the nanoparticles comprising the at least one long-chain RNA and the at least one cationic or polycationic compound from the reactor.