CPC A61K 39/145 (2013.01) [A61K 9/5169 (2013.01); A61K 9/5192 (2013.01); A61K 39/12 (2013.01); A61K 47/646 (2017.08); A61K 47/6455 (2017.08); A61K 2039/55555 (2013.01); C12N 2760/16134 (2013.01)] | 35 Claims |
1. A method for producing a liquid composition comprising a nanoparticle comprising at least one long-chain RNA and at least one cationic or polycationic compound,
wherein the method comprises the steps of:
(a) providing a first liquid composition comprising the at least one long-chain RNA that comprises from 200 to 15,000 nucleotides,
(b) providing a second liquid composition comprising the at least one cationic or polycationic compound,
(c) introducing the first liquid composition and the second liquid composition into at least one reactor, wherein the first liquid composition and/or the second liquid composition are introduced at a flow rate of at least 5 mL/minute, and
(d) recovering the product liquid composition comprising the nanoparticles comprising the at least one long-chain RNA and the at least one cationic or polycationic compound from the reactor.
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