US 11,744,868 B1
Inonotus obliquus dextran, preparation method and application thereof
Qi Wang, Changchun (CN); Yu Yang, Changchun (CN); Yang Liu, Changchun (CN); and Ling Su, Changchun (CN)
Assigned to JILIN AGRICULTURAL UNIVERSITY, Changchun (CN)
Filed by Jilin Agricultural University, Changchun (CN)
Filed on Feb. 8, 2023, as Appl. No. 18/107,255.
Claims priority of application No. 202210305707.8 (CN), filed on Mar. 25, 2022.
Int. Cl. A61K 36/07 (2006.01); A61K 31/721 (2006.01); A61P 35/00 (2006.01); A61P 1/18 (2006.01)
CPC A61K 36/07 (2013.01) [A61K 31/721 (2013.01); A61P 1/18 (2018.01); A61P 35/00 (2018.01); A61K 2236/331 (2013.01); A61K 2236/333 (2013.01); A61K 2236/53 (2013.01)] 3 Claims
 
1. An application of an Inonotus Obliquus dextran in preparing drugs for treating human pancreatic cancer, comprising a method for preparing the Inonotus Obliquus dextran as follows:
(1) extracting Inonotus Obliquus crude polysaccharide from Inonotus Obliquus fruiting body by a water-extraction and alcohol-precipitation method;
(2) deproteinizing the Inonotus Obliquus crude polysaccharide in the step (1) by solvent extraction, followed by separation of components with molecular weight above 3,500 Dalton (Da) by dialysis to obtain a deproteinized crude sugar;
(3) purifying the deproteinized crude sugar by a DEAE-52 (Diethylaminoethyl) cellulose column to obtain a purified Inonotus Obliquus polysaccharide; and
(4) placing the purified Inonotus Obliquus polysaccharide on a Sephadex G-100 gel column and eluting the purified Inonotus Obliquus polysaccharide with distilled water at a controlled flow rate of 0.5 milliliter per minute (mL/min) to obtain the Inonotus Obliquus dextran;
wherein the purifying in the step (3) specifically comprises: dissolving the deproteinized crude sugar in water, followed by loading onto the DEAE-52 cellulose chromatographic column; then performing fractional elution with distilled water, 0.05 mole per liter (mol/L) NaCl, 0.1 mol/L NaCl and 0.2 mol/L NaCl respectively under a controlled flow rate of 1.0 mL/min, separately collecting eluents and combining polysaccharide elution sites to obtain purified Inonotus Obliquus polysaccharide; and
the Inonotus Obliquus dextran is in a molecular weight of 6.886 Kilodalton (kDa).