US 11,744,243 B2
Systems and methods for extraction and cryopreservation of bone marrow
Erik J. Woods, Carmel, IN (US); Brian H. Johnstone, Fishers, IN (US); Dongsheng Gu, Indianapolis, IN (US); Aubrey Marie Sherry, Carmel, IN (US); and Kelsey Gwen Musall, Avon, IN (US)
Assigned to OSSIUM HEALTH, INC., San Francisco, CA (US)
Filed by OSSIUM HEALTH, INC., San Francisco, CA (US)
Filed on Mar. 1, 2022, as Appl. No. 17/684,259.
Application 17/684,259 is a continuation of application No. PCT/US2021/055081, filed on Oct. 14, 2021.
Claims priority of provisional application 63/168,178, filed on Mar. 30, 2021.
Claims priority of provisional application 63/130,255, filed on Dec. 23, 2020.
Claims priority of provisional application 63/110,571, filed on Nov. 6, 2020.
Claims priority of provisional application 63/091,890, filed on Oct. 14, 2020.
Prior Publication US 2022/0183275 A1, Jun. 16, 2022
Int. Cl. A01N 1/02 (2006.01)
CPC A01N 1/0284 (2013.01) [A01N 1/0221 (2013.01); A01N 1/0252 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for processing a biological sample comprising cells, the method comprising:
(a) generating a transplantable unit of the biological sample comprising cells, wherein the transplantable unit comprises a first volume of the cells at a first concentration;
(b) generating a surrogate unit of the biological sample comprising cells, wherein the surrogate unit comprises a second volume of the cells at a second concentration,
wherein the second volume is less than the first volume and the second concentration is no more than 30% different from the first concentration;
(c) cooling the cells of the transplantable unit and the cells of the surrogate unit at a common optimized rate which balances damage from intracellular ice formation with damage from extracellular ice formation; and
(d) storing the transplantable unit and the surrogate unit in the same long-term storage container which exposes the cells of the transplantable unit and the cells of the surrogate unit to similar conditions over a long-term storage duration
thereby providing an equivalent survival and viability for cells from the transplantable unit and for cells from the surrogate unit including a post-thaw cell proliferation rate of the cells of the surrogate unit which is no more than 30% different from a post-thaw cell proliferation rate of the cells of the transplantable unit such that the surrogate unit accurately represents the transplant unit.