US 11,733,152 B2
Microfluidic system with combined electrical and optical detection for high accuracy particle sorting and methods thereof
Hany Nassef, Hayward, CA (US); Karthik Balakrishnan, Hayward, CA (US); Anand Kesavaraju, Hayward, CA (US); Vincent Tuminelli, Hayward, CA (US); and George Anwar, Hayward, CA (US)
Assigned to NODEXUS INC., San Jose, CA (US)
Filed by NODEXUS INC., Hayward, CA (US)
Filed on May 27, 2022, as Appl. No. 17/826,771.
Application 17/826,771 is a continuation of application No. 16/483,922, granted, now 11,579,073, previously published as PCT/US2018/017036, filed on Feb. 6, 2018.
Claims priority of provisional application 62/456,069, filed on Feb. 7, 2017.
Prior Publication US 2022/0291111 A1, Sep. 15, 2022
Int. Cl. G01N 15/14 (2006.01); B01L 3/00 (2006.01); B07C 5/342 (2006.01); B07C 5/344 (2006.01); G01N 15/10 (2006.01)
CPC G01N 15/1484 (2013.01) [B01L 3/502715 (2013.01); B01L 3/502761 (2013.01); B07C 5/344 (2013.01); B07C 5/3425 (2013.01); G01N 15/1459 (2013.01); B01L 2200/0652 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0874 (2013.01); B01L 2400/06 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/1081 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A particle sorting cartridge, comprising:
a sorting region comprising an intersection of:
a first microfluidic channel leading from a sensing region;
a second microfluidic channel leading from a trigger inlet and substantially perpendicular to the first microfluidic channel at the intersection;
a default branch channel leading away from the intersection and operably associated with the first microfluidic channel at the intersection; and
a selected branch channel leading away from the intersection at an angle acute to the default branch channel at the intersection, wherein the selected branch channel leads to a carrier channel connecting a carrier inlet to a sample collection outlet;
wherein the first microfluidic channel, the second microfluidic channel, and the default branch channel have substantially the same diameter at the intersection and the selected branch channel has a narrower diameter than the first microfluidic channel at the intersection; and
wherein the sorting region is configured to direct a sample liquid from the sensing region to the default branch channel in absence of a threshold flow of trigger fluid from the trigger inlet through the second microfluidic channel and further configured to direct the sample liquid from the sensing region to the selected branch channel during application of the threshold flow of trigger fluid from the trigger inlet through the second microfluidic channel.