Nathan Tanner, West Newbury, MA (US); Yinhua Zhang, North Reading, MA (US); Eric Hunt, Danvers, MA (US); Gregory Patton, Peabody, MA (US); Guoping Ren, Danvers, MA (US); Zhiru Li, Lexington, MA (US); Andrew Barry, Ipswich, MA (US); Nicole Nichols, Reading, MA (US); Catherine B. Poole, Medford, MA (US); Harriet M. Strimpel, Manchester, MA (US); Ivan R. Correa, Jr., Hamilton, MA (US); Clotilde Carlow, Ipswich, MA (US); Esta Slayton, Epping, NH (US); and Thomas C. Evans, Jr., Topsfield, MA (US)
Assigned to New England Biolabs, Inc., Ipswich, MA (US)
Filed by New England Biolabs, Inc., Ipswich, MA (US)
Filed on Sep. 28, 2022, as Appl. No. 17/936,084.
Application 17/936,084 is a continuation in part of application No. 17/699,950, filed on Mar. 21, 2022, granted, now 11,525,166.
Application 17/699,950 is a continuation of application No. 17/406,959, filed on Aug. 19, 2021, granted, now 11,345,970.
Application 17/406,959 is a continuation in part of application No. 17/221,451, filed on Apr. 2, 2021, granted, now 11,155,887.
Application 17/221,451 is a continuation of application No. 17/178,395, filed on Feb. 18, 2021, granted, now 11,492,673.
Application 17/178,395 is a continuation of application No. 17/122,979, filed on Dec. 15, 2020, granted, now 11,008,629.
Application 17/122,979 is a continuation of application No. 16/938,575, filed on Jul. 24, 2020, granted, now 10,968,493, issued on Apr. 6, 2021.
Claims priority of provisional application 63/165,465, filed on Mar. 24, 2021.
Claims priority of provisional application 63/106,120, filed on Oct. 27, 2020.
Claims priority of provisional application 63/068,564, filed on Aug. 21, 2020.
Claims priority of provisional application 63/048,556, filed on Jul. 6, 2020.
Claims priority of provisional application 63/027,216, filed on May 19, 2020.
Claims priority of provisional application 63/022,303, filed on May 8, 2020.
Claims priority of provisional application 63/013,442, filed on Apr. 21, 2020.
Claims priority of provisional application 63/001,909, filed on Mar. 30, 2020.
Claims priority of provisional application 62/988,696, filed on Mar. 12, 2020.
Prior Publication US 2023/0048863 A1, Feb. 16, 2023
(a) at least one set of oligonucleotide primers for amplifying a template sequence by Loop-mediated isothermal amplification (LAMP), the set comprising a pair of inner primers, each inner primer comprising, a sequence complementary to a primer binding site of the template sequence and a tail comprising a sequence that is downstream of the 3′ end of the primer binding site of the template;
(b) a strand displacing DNA polymerase, wherein the strand displacing DNA polymerase is in a master mix, the master mix further comprising uracil deglycosylase; and
(c) a nucleic acid probe wherein the probe has (a) at least one label attached to the end of the probe, wherein the at least one label optionally comprises a labelled nucleotide; (b) a sequence that is complementary to a target DNA at a position that is distinct from the primer binding sites; and (c) one or more LNA nucleotides.
