US 11,732,312 B2
Molecular marker of plasmodium falciparum artemisinin resistance
Frederic Ariey, Choisy le Roi (FR); Odile Puijalon, Issy les Moulineaux (FR); Didier Menard, Le Gavre (FR); Francoise Benoit-Vical, Castanet Tolosan (FR); Johann Beghain, Conflans Sainte-honorine (FR); Benoit Witkowski, Montauban (FR); Jean-Christophe Barale, Vanves (FR); Christiane Bouchier, Paris (FR); and Nimol Khim, Kandal (KH)
Assigned to INSTITUT PASTEUR, Paris (FR); CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR); and INSTITUT PASTEUR DU CAMBODGE, Phnom Penh (KH)
Filed by INSTITUT PASTEUR, Paris (FR); INSTITUT PASTEUR DU CAMBODGE, Phnom Penh (KH); and CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR)
Filed on Dec. 16, 2020, as Appl. No. 17/124,478.
Application 17/124,478 is a division of application No. 15/036,064, granted, now 10,900,093, previously published as PCT/IB2014/002910, filed on Nov. 14, 2014.
Claims priority of provisional application 61/904,651, filed on Nov. 15, 2013.
Claims priority of provisional application 62/062,439, filed on Oct. 10, 2014.
Prior Publication US 2021/0198755 A1, Jul. 1, 2021
Int. Cl. C12Q 1/6893 (2018.01)
CPC C12Q 1/6893 (2013.01) [C12Q 2600/118 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/16 (2013.01); G01N 2333/445 (2013.01); Y02A 50/30 (2018.01)] 14 Claims
 
1. A kit for detecting a Plasmodium infection or for genotyping Plasmodium, comprising:
a probe for detection of at least one non-synonymous SNP within a mutated K-13 propeller nucleic acid that encodes a mutated K13 propeller protein;
wherein the probe comprises a fluorescent, radioactive or enzymatic label;
wherein the protein mutation is a difference in an amino acid residue resulting from said non-synonymous SNP; and
wherein the at least one non-synonymous SNP is selected from:
G449A ggt/gct,
N458Y aat/tat,
A481V gct/gtt,
Y493H tac/cac,
R539T aga/aca,
I543T att/act,
P553L ccg/ctg,
R561H cgt/cat,
V568G gtg/ggg,
P574L cct/ctt,
C580Y tgt/tat, and
D584V gat/gtt.