US 11,732,298 B2
Methods for biological sample processing and analysis
Gilad Almogy, Palo Alto, CA (US); Kristopher Barbee, Pleasanton, CA (US); and Nathan Beckett, Oakland, CA (US)
Assigned to Ultima Genomics, Inc., Newark, CA (US)
Filed by Ultima Genomics, Inc., Newark, CA (US)
Filed on May 20, 2019, as Appl. No. 16/416,856.
Application 16/416,856 is a continuation of application No. 15/974,364, filed on May 8, 2018, granted, now 10,344,328.
Claims priority of provisional application 62/664,049, filed on Apr. 27, 2018.
Claims priority of provisional application 62/623,743, filed on Jan. 30, 2018.
Claims priority of provisional application 62/588,139, filed on Nov. 17, 2017.
Prior Publication US 2019/0271038 A1, Sep. 5, 2019
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6874 (2018.01); C12Q 1/6809 (2018.01); C12Q 1/6825 (2018.01); B01J 19/00 (2006.01); B01L 3/00 (2006.01); G01N 33/543 (2006.01); C12Q 1/6834 (2018.01)
CPC C12Q 1/6874 (2013.01) [B01J 19/0046 (2013.01); B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); C12Q 1/6809 (2013.01); C12Q 1/6825 (2013.01); B01J 2219/00536 (2013.01); B01J 2219/00596 (2013.01); B01J 2219/00605 (2013.01); B01J 2219/00689 (2013.01); B01J 2219/00702 (2013.01); B01J 2219/00722 (2013.01); B01L 3/5023 (2013.01); B01L 2200/026 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/0654 (2013.01); B01L 2400/0409 (2013.01); C12Q 1/6834 (2013.01); G01N 33/54366 (2013.01)] 30 Claims
OG exemplary drawing
 
1. A method for processing a biological analyte, comprising:
(a) directing a solution comprising a plurality of probes from a first location of a substrate to a second location of said substrate, wherein said first location is different than said second location, wherein said biological analyte is immobilized adjacent to said substrate at said first location or said second location, and wherein said directing is performed in absence of flowing said solution through a flow cell channel;
(b) coupling at least one probe of said plurality of probes to said biological analyte; and
(c) detecting, by providing an excitation light down to said substrate and using a detector comprising an optical imaging objective disposed above said substrate, wherein said optical imaging objective is at least partially immersed in a fluid in contact with said substrate, one or more signals or signal changes from said biological analyte having said at least one probe coupled thereto.