US 11,732,293 B2
Biomarker detection from fluid samples
Rashid Bashir, Champaign, IL (US); and Anurup Ganguli, Champaign, IL (US)
Assigned to The Board of Trustees of the University of Illinois, Urbana, IL (US)
Appl. No. 16/649,894
Filed by The Board of Trustees of the University of Illinois, Urbana, IL (US)
PCT Filed Oct. 5, 2018, PCT No. PCT/US2018/054634
§ 371(c)(1), (2) Date Mar. 23, 2020,
PCT Pub. No. WO2019/071142, PCT Pub. Date Apr. 11, 2019.
Claims priority of provisional application 62/569,119, filed on Oct. 6, 2017.
Prior Publication US 2020/0263244 A1, Aug. 20, 2020
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6848 (2018.01); B01L 3/00 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6851 (2018.01); C12Q 1/689 (2018.01); G01N 27/414 (2006.01); B03C 1/01 (2006.01); B03C 1/28 (2006.01); B03C 1/30 (2006.01)
CPC C12Q 1/6848 (2013.01) [B01L 3/5027 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/689 (2013.01); C12Q 1/6851 (2013.01); G01N 27/4145 (2013.01); B01L 2200/0689 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/0893 (2013.01); B01L 2300/161 (2013.01); B03C 1/01 (2013.01); B03C 1/288 (2013.01); B03C 1/30 (2013.01); B03C 2201/18 (2013.01); B03C 2201/26 (2013.01)] 40 Claims
 
1. A method of detecting one or more target analytes from a liquid sample, the method comprising the steps of:
applying a liquid sample to a substrate, wherein the substrate has an array of wells;
drying the liquid sample to generate a plurality of dried sample islands, with each sample island confined to a unique well;
applying a liquid having a reagent suspended in a liquid buffer to each well, wherein the reagent is used for nucleic acid detection;
eluting a nucleic acid from the dried sample islands with a liquid phase having the applied reagent;
detecting a target nucleic acid if present in a dried sample island by a bi-phasic reaction, that occurs in both the dried sample islands and in the liquid having the reagent suspended in the liquid buffer, wherein the detecting is by electrical or optical detection;
thereby detecting the one or more target analytes from the liquid biological sample.