US 11,732,291 B2
Asymmetric hairpin target capture oligomers
James Carlson, San Diego, CA (US); Reinhold Pollner, San Diego, CA (US); and Steven T. Brentano, San Diego, CA (US)
Assigned to GEN-PROBE INCORPORATED, San Diego, CA (US)
Filed by GEN-PROBE INCORPORATED, San Diego, CA (US)
Filed on Apr. 15, 2020, as Appl. No. 16/849,857.
Application 16/849,857 is a division of application No. 14/376,128, granted, now 10,655,165, previously published as PCT/US2013/024499, filed on Feb. 1, 2013.
Claims priority of provisional application 61/593,829, filed on Feb. 1, 2012.
Prior Publication US 2020/0248246 A1, Aug. 6, 2020
Int. Cl. C07H 21/04 (2006.01); C12Q 1/6834 (2018.01); C12Q 1/6816 (2018.01)
CPC C12Q 1/6834 (2013.01) [C12Q 1/6816 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A target capture oligomer comprising a first stem segment and a second stem segment comprising complementary segments of polyA and polyT differing in length by at least five nucleobases flanking a target-binding segment complementary to a target nucleic acid, wherein under hybridizing conditions:
in the absence of the target nucleic acid, the target capture oligomer forms a stem-loop structure such that, in the stem-loop structure, intramolecular hybridization of the first stem segment and the second stem segment forms the stem, and the target-binding segment forms the loop; and
in the presence of the target nucleic acid, the target-binding segment hybridizes to the target nucleic acid separating or keeping separate the first stem segment and the second stem segment and resulting in the first stem segment being accessible to hybridize to a complementary immobilized probe.