CPC C12N 15/86 (2013.01) [C12N 9/22 (2013.01); C12N 15/113 (2013.01); C07K 2319/80 (2013.01); C12Y 305/04005 (2013.01)] | 32 Claims |
1. A cytidine deaminase comprising an amino acid sequence that is at least 90% identical to amino acid residues 3-229 of SEQ ID NO: 2, wherein the cytidine deaminase comprises one or more mutations selected from the group consisting of E4X1, V10X2, E31X3, Y40X4, E95X5, H109X6, H122X7, D124X8, R154X10, N158X11, A165X12, P201X13, F205X14, and I208X15 relative to SEQ ID NO: 2, wherein X1, X3, and X5 are any amino acid other than E, X2 is any amino acid other than V, X4 is any amino acid other than Y, X6 is N, X7 is any amino acid other than H, X8 is any amino acid other than D, X10 is any amino acid other than R, X11 is any amino acid other than N, X12 is any amino acid other than A, X13 is any amino acid other than P, X14 is any amino acid other than F, and X15 is any amino acid other than I.
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