	US 11,732,254 B2
	Size selection purification using a thermoplastic silica nanomaterial
Kelvin Jeng-Fang Liu, Baltimore, MD (US); John Duncan Kilburn, Baltimore, MD (US); and Jeffrey Michael Burke, Baltimore, MD (US)
Assigned to Pacific Biosicences of California, Inc., Menlo Park, CA (US)
Appl. No. 16/627,578
Filed by CIRCULOMICS, INC., Baltimore, MD (US)
PCT Filed Jun. 29, 2018, PCT No. PCT/US2018/040324 § 371(c)(1), (2) Date Dec. 30, 2019, PCT Pub. No. WO2019/006321, PCT Pub. Date Jan. 3, 2019.
Claims priority of provisional application 62/527,659, filed on Jun. 30, 2017.
Prior Publication US 2020/0255822 A1, Aug. 13, 2020
Int. Cl. C12N 15/10 (2006.01); B01J 20/10 (2006.01)

CPC C12N 15/1017 (2013.01) [B01J 20/10 (2013.01)]

31 Claims

1. A method of size selecting nucleic acids, the method comprising:

a. adding a precipitation solution comprising water, salt, and polyvinylpyrrolidone (PVP) and/or Ficoll to a nucleic acid-containing sample to produce a sample-solution;

b. allowing nucleic acids of one or more selected sizes in the nucleic acid-containing sample to precipitate driven by the PVP and/or the Ficoll to produce precipitated nucleic acids; and,

c. size selecting nucleic acids by separating the precipitated nucleic acids from the nucleic acid remaining in solution,

wherein the method lacks a separate magnetic bead purification step.