US 11,732,229 B2
Modular organ microphysiological system with integrated pumping, leveling, and sensing
Linda Griffith, Cambridge, MA (US); David Trumper, Cambridge, MA (US); Collin Edington, San Mateo, CA (US); Gaurav Rohatgi, Boston, MA (US); Duncan Freake, Boston, MA (US); Luis Soenksen, Boston, MA (US); and Brij Mohan Bhushan, Cambridge, MA (US)
Assigned to MASSACHUSETTS INSTITUTE OF TECHNOLOGY, Cambridge, MA (US)
Filed by Massachusetts Institute of Technology, Cambridge, MA (US)
Filed on Nov. 20, 2020, as Appl. No. 17/100,542.
Application 17/100,542 is a division of application No. 15/425,858, filed on Feb. 6, 2017, granted, now 10,876,088.
Claims priority of provisional application 62/359,567, filed on Jul. 7, 2016.
Claims priority of provisional application 62/291,102, filed on Feb. 4, 2016.
Prior Publication US 2021/0139829 A1, May 13, 2021
Int. Cl. C12M 1/32 (2006.01); F04B 23/04 (2006.01); F04B 43/12 (2006.01); B01L 3/00 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); F04B 43/04 (2006.01); F04B 23/06 (2006.01); F04B 19/00 (2006.01)
CPC C12M 23/12 (2013.01) [B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); B01L 3/502738 (2013.01); C12M 25/14 (2013.01); C12M 29/10 (2013.01); F04B 19/006 (2013.01); F04B 23/04 (2013.01); F04B 23/06 (2013.01); F04B 43/043 (2013.01); F04B 43/12 (2013.01); B01L 2200/0621 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/0887 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/0457 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0655 (2013.01); B01L 2400/086 (2013.01)] 23 Claims
OG exemplary drawing
 
1. A fluidic multi-well device comprising:
a first plate comprising:
two or more wells comprising a three-dimensional space in each well defined by a bottom surface and a circumferential wall; and
an inlet and an outlet in each well;
a network of fluid paths providing fluid connectivity between at least two of the wells through the inlet and the outlet of each of the two wells;
wherein
the first and second wells are cell culture wells,
wherein a first well comprises a first transwell insert having a porous basal surface for attachment of cells in the first transwell, and a second well comprising a second transwell insert having a porous basal surface for attachment of cells in the second transwell, and
the first well has fluid paths for circulation of culture media in the first well to the basal side of the first transwell and the second well has fluid paths for circulation of culture media through the porous basal surface of the second transwell and into the first well.