CPC C09B 69/109 (2013.01) [C08G 61/10 (2013.01); C08G 61/12 (2013.01); C09B 69/00 (2013.01); C09B 69/101 (2013.01); C09B 69/102 (2013.01); G01N 33/582 (2013.01); C08G 2261/135 (2013.01); C08G 2261/143 (2013.01); C08G 2261/145 (2013.01); C08G 2261/1424 (2013.01); C08G 2261/1452 (2013.01); C08G 2261/1644 (2013.01); C08G 2261/312 (2013.01); C08G 2261/334 (2013.01); C08G 2261/344 (2013.01); C08G 2261/522 (2013.01); C08G 2261/90 (2013.01); G01N 33/533 (2013.01); G01N 33/542 (2013.01)] | 19 Claims |
1. A method of evaluating a sample for the presence of a target analyte, the method comprising:
(a) contacting the sample with a polymeric dye conjugate that specifically binds the target analyte to produce a labelling composition contacted sample, wherein the polymeric dye conjugate comprises:
(i) a polymeric dye having a deep ultraviolet excitation spectrum and comprising: a segment of π-conjugated co-monomers; and a conjugation-modifying repeat unit; and
(ii) a specific binding member covalently linked to the polymeric dye; and
(b) assaying the labelling composition contacted sample for the presence of a polymeric dye conjugate-target analyte binding complex to evaluate whether the target analyte is present in the sample, wherein the conjugation-modifying repeat unit comprises the structure:
wherein:
Z is selected from a covalent bond, a saturated atom, a saturated group and a functional group, with the proviso that when Z is a covalent bond at least two of R22, R23, R25 and R28 are not H; R21-R28 are independently selected from H, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, alkoxy, substituted alkoxy, hydroxy, cyano, halogen, sulfonate, sulfonamide, substituted sulfonamide, carboxy, carboxyamide, substituted carboxyamide, and a water solubilizing group, and wherein one or more of R22, R23, R25 and R28 is optionally cyclically linked to Z to provide a benzo-fused carbocycle or heterocycle ring; and each * is a site for covalent attachment to the backbone of the polymeric dye.
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