US 11,732,014 B2
Modulation of Solanaceae fruit ripening
Charles Baxter, Bracknell (GB); Yu Pan, Loughborough (GB); Thomas Charles Hodgman, Loughborough (GB); Graham Barron Seymour, Loughborough (GB); Rebecca Cade, Durham, NC (US); Henricus Johannes Van Wijk, Saint-Saveur (FR); Glyn Bradley, Londonderry (IE); Laurent Grivet, Saint-Sauveur (FR); Laurie Boyden, Stanton, MN (US); Graham Ball, Nottingham (GB); and Paul Fraser, Egham (GB)
Assigned to Syngenta Participations AG, Basel (CH)
Appl. No. 13/825,203
Filed by Charles Baxter, Bracknell (GB); Yu Pan, Loughborough (GB); Thomas Charles Hodgman, Loughborough (GB); Graham Barron Seymour, Loughborough (GB); Rebecca Cade, Durham, NC (US); Henricus Johannes Van Wijk, Saint-Saveur (FR); Glyn Bradley, Londonderry (IE); Laurent Grivet, Saint-Sauveur (FR); Laurie Boyden, Stanton, MN (US); Graham Ball, Nottingham (GB); and Paul Fraser, Egham (GB)
PCT Filed Sep. 27, 2011, PCT No. PCT/EP2011/066773
§ 371(c)(1), (2), (4) Date Nov. 20, 2013,
PCT Pub. No. WO2012/041856, PCT Pub. Date Apr. 5, 2012.
Claims priority of application No. 10183748 (EP), filed on Sep. 30, 2010.
Prior Publication US 2014/0137296 A1, May 15, 2014
Int. Cl. A01H 6/82 (2018.01); C12N 15/82 (2006.01); C07K 14/415 (2006.01)
CPC C07K 14/415 (2013.01) [A01H 6/822 (2018.05); C12N 15/825 (2013.01); C12N 15/8249 (2013.01); C12N 15/8261 (2013.01)] 2 Claims
 
1. A method of manipulating the speed of ripening or the pigment content of a pepper fruit produced by a pepper plant by genetically modifying a pepper APPR2 gene of SEQ ID NO: 2 or SEQ ID NO: 14 in said pepper plant, the method comprising:
(a) generating by TILLING variant gene products of a pepper APPR2 gene of SEQ ID NO: 2 or SEQ ID NO: 14 in a plurality of pepper plants to produce a plurality of genetically modified pepper plants; and
(b) selecting a pepper plant from the plurality of genetically modified pepper plants of a) with a decreased speed of ripening or decreased pigment content in pepper fruits produced by said plant as compared with a pepper plant having the pepper APPR2 gene of SEQ ID NO: 2 or SEQ ID NO: 14, thereby manipulating the speed of ripening or the pigment content of a pepper fruit produced by said genetically modified pepper plant.