US 11,732,011 B2
Self-assembling protein nanostructures displaying paramyxovirus and/or pneumovirus F proteins and their use
Neil P. King, Seattle, WA (US); David Baker, Seattle, WA (US); Brooke Fiala, Seattle, WA (US); Lance Joseph Stewart, Seattle, WA (US); Laurent Perez, Bellinzona (CH); Antonio Lanzavecchia, Bellinzona (CH); and Jessica Marcandalli, Bellinzona (CH)
Assigned to University of Washington, Seattle, WA (US); and Institute for Research in Biomedicine, Bellinzona (CH)
Filed by UNIVERSITY OF WASHINGTON, Seattle, WA (US); and INSTITUTE FOR RESEARCH IN BIOMEDICINE, Bellinzona (CH)
Filed on Nov. 10, 2021, as Appl. No. 17/523,174.
Application 17/523,174 is a division of application No. 16/500,331, granted, now 11,192,926, previously published as PCT/US2018/025880, filed on Apr. 3, 2018.
Claims priority of provisional application 62/481,331, filed on Apr. 4, 2017.
Prior Publication US 2022/0169681 A1, Jun. 2, 2022
Int. Cl. C12N 7/00 (2006.01); C12N 1/15 (2006.01); C12N 15/45 (2006.01); A61K 39/12 (2006.01); A61K 39/155 (2006.01); C07K 14/005 (2006.01); A61P 31/14 (2006.01); B82Y 5/00 (2011.01); A61K 39/00 (2006.01)
CPC C07K 14/005 (2013.01) [A61K 39/12 (2013.01); A61P 31/14 (2018.01); B82Y 5/00 (2013.01); C12N 7/00 (2013.01); A61K 2039/575 (2013.01); C07K 2319/735 (2013.01); C12N 2760/18022 (2013.01); C12N 2760/18322 (2013.01); C12N 2760/18522 (2013.01)] 30 Claims
 
1. A nanostructure, comprising:
(a) a plurality of first assemblies, each first assembly comprising a plurality of identical first polypeptides, wherein the first polypeptides comprise polypeptides having at least 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identity to the amino acid sequence of a polypeptide selected from the group consisting of SEQ ID NOS:1-51;
(b) a plurality of second assemblies, each second assembly comprising a plurality of identical second polypeptides, wherein the second polypeptides comprise polypeptides having at least 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identity to the amino acid sequence of a polypeptide selected from the group consisting of SEQ ID NOS:1-51,
wherein the second polypeptide differs from the first polypeptide;
wherein the plurality of first assemblies non-covalently interact with the plurality of second assemblies to form a nanostructure; and
wherein the nanostructure displays multiple copies of one or more paramyxovirus and/or pneumovirus F proteins, or antigenic fragments thereof, on an exterior of the nanostructure.