US 11,732,009 B2
Activity sensor with tunable analyte
Sangeeta Bhatia, Lexington, MA (US); Gabriel Kwong, Atlanta, GA (US); Eric Huang, Lexington, MA (US); Sirshendu Roopom Banerjee, Lexington, MA (US); Andrew Warren, Cambridge, MA (US); and Sophie Cazanave, Cambridge, MA (US)
Assigned to GLYMPSE BIO, INC., Cambridge, MA (US)
Filed by GLYMPSE BIO, INC., Cambridge, MA (US)
Filed on Mar. 15, 2019, as Appl. No. 16/355,178.
Claims priority of provisional application 62/682,492, filed on Jun. 8, 2018.
Prior Publication US 2019/0376113 A1, Dec. 12, 2019
Int. Cl. A61K 47/65 (2017.01); C12Q 1/37 (2006.01); A61K 47/60 (2017.01); A61K 38/00 (2006.01); C07K 14/00 (2006.01); G01N 33/68 (2006.01); A61K 47/10 (2017.01); C07K 11/02 (2006.01)
CPC C07K 14/00 (2013.01) [A61K 47/10 (2013.01); A61K 47/60 (2017.08); A61K 47/65 (2017.08); C07K 11/02 (2013.01); C12Q 1/37 (2013.01); G01N 33/68 (2013.01); A61K 38/00 (2013.01); G01N 2333/96494 (2013.01); G01N 2800/085 (2013.01)] 12 Claims
OG exemplary drawing
 
1. An activity sensor comprising:
a carrier comprising a polyethylene glycol (PEG) scaffold comprised of covalently linked PEG subunits; and
at least two distinct detectable peptide reporters, wherein each of said at least two distinct detectable peptide reporters is linked to the carrier by a cleavable peptide linker containing a cleavage site for one of at least two different enzymes, wherein the activity sensor reports differential enzyme activity among the at least two different enzymes by releasing the detectable peptide reporters upon cleavage of the cleavable peptide linker by the at least two different enzymes;
wherein each of the detectable peptide reporters is coupled to a biocompatible polymer,
wherein the biocompatible polymer is configured to stabilize and protect each of the coupled detectable peptide reporters from immune detection before and after cleavage of the cleavable peptide linker.