CPC C07K 1/22 (2013.01) [C07K 16/00 (2013.01)] | 20 Claims |
1. A method of isolating a first protein from a blood product, comprising:
mixing the blood product comprising the first protein and a second protein with a lipid or lipoprotein adsorbing particulate and an organic salt to generate a suspension comprising a first precipitate and a first supernatant, wherein the first precipitate comprises a precipitated second protein and the lipid or lipoprotein adsorbing material complexed with a lipid or a lipoprotein present in the blood product;
separating the suspension by centrifugation to generate a first stream comprising the first supernatant, a second stream comprising the precipitated second protein, and third stream comprising the lipid or lipoprotein adsorbing material complexed with a lipid or a lipoprotein present in the blood product;
applying the first supernatant to a chromatography media to obtain an effluent comprising the first protein from the chromatography media; and
recovering the second protein from the second stream,
wherein the concentration of the organic salt is sufficient to precipitate a first non-protein of interest protein from the blood product.
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