US 11,732,004 B2
Compositions and methods for simplified high efficiency isolation of proteins
Eugene Zurlo, Charleston, SC (US); Dennis Curtin, Plattsburgh, NY (US); Klaus Peter Radtke, Charleston, SC (US); and Kurt L. Brillhart, Mission Viejo, CA (US)
Assigned to Plasma Technologies, LLC, Charleston, SC (US)
Filed by PLASMA TECHNOLOGIES, LLC, Charleston, SC (US)
Filed on Apr. 8, 2021, as Appl. No. 17/225,934.
Claims priority of provisional application 63/008,365, filed on Apr. 10, 2020.
Prior Publication US 2021/0317163 A1, Oct. 14, 2021
Int. Cl. C07K 1/22 (2006.01); C07K 16/00 (2006.01); C07K 1/16 (2006.01); C07K 16/06 (2006.01); C07K 1/30 (2006.01)
CPC C07K 1/22 (2013.01) [C07K 16/00 (2013.01)] 20 Claims
 
1. A method of isolating a first protein from a blood product, comprising:
mixing the blood product comprising the first protein and a second protein with a lipid or lipoprotein adsorbing particulate and an organic salt to generate a suspension comprising a first precipitate and a first supernatant, wherein the first precipitate comprises a precipitated second protein and the lipid or lipoprotein adsorbing material complexed with a lipid or a lipoprotein present in the blood product;
separating the suspension by centrifugation to generate a first stream comprising the first supernatant, a second stream comprising the precipitated second protein, and third stream comprising the lipid or lipoprotein adsorbing material complexed with a lipid or a lipoprotein present in the blood product;
applying the first supernatant to a chromatography media to obtain an effluent comprising the first protein from the chromatography media; and
recovering the second protein from the second stream,
wherein the concentration of the organic salt is sufficient to precipitate a first non-protein of interest protein from the blood product.