US 11,731,982 B2
Biotin derivatives
Lai-Qiang Ying, Eugene, OR (US); Bruce Branchaud, Eugene, OR (US); Yu-Zhong Zhang, Eugene, OR (US); and Stephen Yue, Eugene, OR (US)
Assigned to LIFE TECHNOLOGIES CORPORATION
Filed by LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed on Mar. 27, 2019, as Appl. No. 16/366,908.
Application 16/366,908 is a division of application No. 15/390,820, filed on Dec. 27, 2016, abandoned.
Application 15/390,820 is a division of application No. 13/881,518, granted, now 9,567,346, previously published as PCT/US2011/058455, filed on Oct. 28, 2011.
Claims priority of provisional application 61/510,949, filed on Jul. 22, 2011.
Claims priority of provisional application 61/501,500, filed on Jun. 27, 2011.
Claims priority of provisional application 61/495,070, filed on Jun. 9, 2011.
Claims priority of provisional application 61/408,210, filed on Oct. 29, 2010.
Prior Publication US 2019/0322681 A1, Oct. 24, 2019
Int. Cl. G01N 33/567 (2006.01); C07D 495/04 (2006.01); C08B 37/00 (2006.01); G01N 33/532 (2006.01); G01N 33/543 (2006.01); B01D 15/38 (2006.01); G01N 33/53 (2006.01); G01N 33/554 (2006.01); G01N 33/569 (2006.01); C07K 16/00 (2006.01); G01N 33/58 (2006.01); C07D 403/12 (2006.01); C07K 1/13 (2006.01); C07K 1/22 (2006.01); C07K 16/42 (2006.01); C09B 11/24 (2006.01); C09K 11/06 (2006.01); C12N 5/0783 (2010.01); G01N 33/533 (2006.01); G01N 33/68 (2006.01); A61K 39/00 (2006.01)
CPC C07D 495/04 (2013.01) [B01D 15/3823 (2013.01); C07D 403/12 (2013.01); C07K 1/13 (2013.01); C07K 1/22 (2013.01); C07K 16/00 (2013.01); C07K 16/42 (2013.01); C08B 37/00 (2013.01); C09B 11/24 (2013.01); C09K 11/06 (2013.01); C12N 5/0636 (2013.01); C12N 5/0637 (2013.01); C12N 5/0638 (2013.01); G01N 33/53 (2013.01); G01N 33/532 (2013.01); G01N 33/533 (2013.01); G01N 33/54353 (2013.01); G01N 33/554 (2013.01); G01N 33/569 (2013.01); G01N 33/58 (2013.01); G01N 33/582 (2013.01); G01N 33/6803 (2013.01); A61K 2039/625 (2013.01); C07B 2200/11 (2013.01); C07K 2317/20 (2013.01); C07K 2317/92 (2013.01); C09K 2211/1007 (2013.01); C09K 2211/1088 (2013.01)] 5 Claims
 
1. A method for separating cells of a first cell type from cells of second cell type, the method comprising:
(a) binding cells of the first cell type to a solid support through an antibody having binding affinity to a cell surface protein of cells of the first cell type, and
(b) separating cells of the first cell type bound to the solid support from cells of the second cell type,
wherein the antibody is bound to the solid support through a biotin derivative/streptavidin linkage, and
wherein the biotin derivative is N3′-ethyl biotin 17 having the following structure:

OG Complex Work Unit Chemistry