	US 11,730,768 B2
	Method for isolation and purification of microvesicles from cell culture supernatants and biological fluids
Evangelos V. Badiavas, Coral Gables, FL (US); Arsalan Q. Shabbir, Miami, FL (US); and Stephen C. Davis, El Portal, FL (US)
Assigned to UNIVERSITY OF MIAMI, Miami, FL (US)
Filed by UNIVERSITY OF MIAMI, Miami, FL (US)
Filed on Oct. 30, 2019, as Appl. No. 16/668,851.
Application 16/668,851 is a continuation of application No. 14/775,382, granted, now 10,500,231, previously published as PCT/US2014/024629, filed on Mar. 12, 2014.
Claims priority of provisional application 61/778,591, filed on Mar. 13, 2013.
Prior Publication US 2020/0163999 A1, May 28, 2020
Int. Cl. A61K 35/28 (2015.01); A61K 35/16 (2015.01); A61K 35/12 (2015.01); A61K 8/98 (2006.01); A61K 35/22 (2015.01); A61Q 19/00 (2006.01); A61Q 19/08 (2006.01)

CPC A61K 35/28 (2013.01) [A61K 8/981 (2013.01); A61K 8/983 (2013.01); A61K 35/12 (2013.01); A61K 35/16 (2013.01); A61K 35/22 (2013.01); A61Q 19/00 (2013.01); A61Q 19/08 (2013.01); A61K 2800/74 (2013.01); A61K 2800/86 (2013.01)]

13 Claims

1. A topical composition for treating wounds comprising an effective amount of an enriched population of intact microvesicles collected from polyethylene glycol precipitation of a mesenchymal stem cell supernatant or a biological fluid;

wherein the mesenchymal stem cells are derived from bone marrow;

wherein the microvesicles range in size from 2 nm to 5000 nm;

wherein the topical composition further comprises a storage solution;

wherein the intact microvesicles result in increased migration of non-proliferating cultured fibroblasts in a cell migration assay when administered to the non-proliferating cultured fibroblasts; and

wherein said effective amount of isolated microvesicles results in regeneration of at least one skin tissue in the skin wound.