	US 11,730,730 B2
	Cell-based screening and compounds that modulate GalNAc-transferases
Adam D. Linstedt, Pittsburgh, PA (US); Lina Song, Gainesville, FL (US); and Collin Bachert, Pittsburgh, PA (US)
Assigned to Carnegie Mellon University, Pittsburgh, PA (US)
Filed by Carnegie Mellon University, Pittsburgh, PA (US)
Filed on May 26, 2021, as Appl. No. 17/330,779.
Application 17/330,779 is a division of application No. 16/369,159, filed on Mar. 29, 2019, granted, now 11,058,675.
Claims priority of provisional application 62/761,585, filed on Mar. 29, 2018.
Prior Publication US 2021/0283124 A1, Sep. 16, 2021
Int. Cl. A61K 31/4709 (2006.01); A61P 13/12 (2006.01); A61P 35/04 (2006.01); G01N 33/50 (2006.01); G01N 33/533 (2006.01); C12Q 1/48 (2006.01)

CPC A61K 31/4709 (2013.01) [A61P 13/12 (2018.01); A61P 35/04 (2018.01); G01N 33/5008 (2013.01); G01N 33/533 (2013.01)]

9 Claims

1. A fluorescent sensor of GalNAc-type O-glycosylation by GalNAc-T1 isozyme comprising a polypeptide comprising, in order, a fluorogen-activating protein (FAP) blocking domain having a sequence of an FAP of SEQ ID NOS: 5-11, a furin cleavage linker having the sequence REDRVTGSYQ (SEQ ID NO: 2), an FAP domain of any one of SEQ ID NOS: 5-11, a transmembrane domain, and, optionally, a fluorescent protein domain.