US 11,730,371 B2
System and method for large field of view, single cell analysis
Moungi G. Bawendi, Cambridge, MA (US); Jorge M. Ferrer, Cambridge, MA (US); W. David Lee, Brookline, MA (US); Lisa F. Marshall, Cambridge, MA (US); and David G. Kirsch, Durham, NC (US)
Assigned to Massachusetts Institute of Technology, Cambridge, MA (US); and Duke University, Durham, NC (US)
Filed by Massachusetts Institute of Technology, Cambridge, MA (US); and Duke University, Durham, NC (US)
Filed on Apr. 15, 2019, as Appl. No. 16/384,355.
Application 16/384,355 is a continuation of application No. 14/616,467, filed on Feb. 6, 2015.
Application 14/616,467 is a continuation of application No. 12/469,395, filed on May 20, 2009, granted, now 8,983,581, issued on Mar. 17, 2015.
Prior Publication US 2019/0239750 A1, Aug. 8, 2019
This patent is subject to a terminal disclaimer.
Int. Cl. A61B 5/00 (2006.01)
CPC A61B 5/0071 (2013.01) [A61B 5/0082 (2013.01); A61B 5/0084 (2013.01); A61B 5/4848 (2013.01); A61B 5/742 (2013.01)] 24 Claims
OG exemplary drawing
 
17. A method for imaging tissue, the method comprising:
contacting a spacer of a medical imaging system with a tissue, wherein a portion of the spacer in contact with the tissue includes a planar surface that extends across a distal end of the spacer;
maintaining a fixed distance between the portion of the spacer in contact with the tissue and an imaging lens, wherein the imaging lens is configured to remain stationary;
emitting an excitation light towards the tissue in substantially a whole field of view of an imaging device at once, wherein the imaging device is optically coupled to the imaging lens;
imaging the tissue in contact with the spacer with the imaging device, wherein imaging the tissue includes simultaneously collecting light fluoresced from the tissue with a plurality of pixels from substantially the whole field of view of the imaging device; and
identifying pixels with light intensities greater than or equal to a predetermined threshold light intensity to identify a cell state of cells imaged by the imaging device.