US 11,726,101 B2
Adrenomedullin assays and methods for determining mature adrenomedullin
Andreas Bergmann, Berlin (DE)
Assigned to SPHINGOTEC GMBH, Hennigsdorf (DE)
Filed by SPHINGOTEC GMBH, Hennigsdorf (DE)
Filed on Jun. 16, 2021, as Appl. No. 17/349,134.
Application 15/344,784 is a division of application No. 14/358,147, granted, now 9,535,060, previously published as PCT/EP2012/072928, filed on Nov. 16, 2012.
Application 17/349,134 is a continuation of application No. 15/344,784, filed on Nov. 7, 2016, granted, now 11,067,586.
Claims priority of application No. 11189447 (EP), filed on Nov. 16, 2011; application No. 11189448 (EP), filed on Nov. 16, 2011; application No. 11189449 (EP), filed on Nov. 16, 2011; application No. 11189450 (EP), filed on Nov. 16, 2011; application No. 11189452 (EP), filed on Nov. 16, 2011; application No. 12160014 (EP), filed on Mar. 16, 2012; application No. 12160015 (EP), filed on Mar. 16, 2012; application No. 12160016 (EP), filed on Mar. 16, 2012; application No. 12160017 (EP), filed on Mar. 16, 2012; application No. 12160018 (EP), filed on Mar. 16, 2012; and application No. 12186449 (EP), filed on Sep. 27, 2012.
Prior Publication US 2021/0311078 A1, Oct. 7, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/53 (2006.01); G01N 33/74 (2006.01)
CPC G01N 33/74 (2013.01) [G01N 2800/26 (2013.01); G01N 2800/52 (2013.01)] 18 Claims
 
1. An in vitro method for therapy follow-up comprising:
forming a complex in a plasma or whole blood sample from a human patient suspected of having sepsis, said complex comprising mature adrenomedullin (ADM) 1-52 and/or mature ADM 1-52-Gly in said sample of plasma or whole blood of said human patient suspected of having sepsis and two antibodies that bind to two different regions within the region of mature adrenomedullin and/or adrenomedullin-Gly that is SEQ ID No. 1 or SEQ ID No. 2 wherein each of said regions comprises at least 4 or 5 amino acids, and
measuring the amount of complex formed and
treating the human patient suspected of having sepsis.