	US 11,726,088 B2
	Sirtuin 4 (SIRT4) lipoamidase activity and uses thereof
Thomas Shenk, Princeton, NJ (US); Todd M. Greco, Langhorne, PA (US); Ileana M. Cristea, Princeton, NJ (US); Rommel A. Mathias, Plainsboro, NJ (US); and Adam Oberstein, Princeton, NJ (US)
Assigned to THE TRUSTEES OF PRINCETON UNIVERSITY, Princeton, NJ (US)
Filed by THE TRUSTEES OF PRINCETON UNIVERSITY, Princeton, NJ (US)
Filed on Aug. 12, 2020, as Appl. No. 16/991,429.
Application 16/991,429 is a continuation of application No. 15/106,932, granted, now 10,788,492, previously published as PCT/US2015/011585, filed on Jan. 15, 2015.
Claims priority of provisional application 62/091,167, filed on Dec. 12, 2014.
Claims priority of provisional application 61/927,799, filed on Jan. 15, 2014.
Prior Publication US 2021/0318312 A1, Oct. 14, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/34 (2006.01); G01N 33/573 (2006.01)

CPC G01N 33/573 (2013.01) [C12Q 1/34 (2013.01); G01N 2333/90212 (2013.01); G01N 2333/91057 (2013.01); G01N 2500/00 (2013.01)]

12 Claims

1. A method of assaying pyruvate dehydrogenase complex (PDHC) activity in a mammalian cell that expresses human Sirtuin 4 (SIRT4) comprising measuring relative abundance of a dihydrolipoyllysine acetyltransferase (DLAT) lipoamide peptide comprising the amino acid sequence TDK[lipoyl]AT in the mammalian cell.