CPC G01N 33/5061 (2013.01) [G01N 33/84 (2013.01); G01N 2500/10 (2013.01)] | 5 Claims |
1. A method for testing drug response of cardiomyocytes, the method comprising:
(a) testing a response of the cardiomyocytes to an added drug in a culture medium under a condition in which a distance from a liquid surface of the culture medium to a bottom surface of a culture vessel contacted by the cardiomyocytes is 5.0 mm or less; or
(b) testing a response of the cardiomyocytes to an added drug immediately after placing the cardiomyocytes in a culture medium under a condition in which a distance from a liquid surface of the culture medium to a bottom surface of a culture vessel contacted by the cardiomyocytes is 5.0 mm or less, wherein
(i) the cell density of the cardiomyocytes in the culture medium is greater than or equal to 2.5×105 cells/cm2, and the distance from the liquid surface of the culture medium to the bottom surface of the culture vessel is 1.5 mm or less; or
(ii) the cell density of the cardiomyocytes in the culture medium is equal to or greater than 1.25×105 cells/cm2, and less than 2.5×105 cells/cm2, and the distance from the liquid surface of the culture medium to the bottom surface of the culture vessel contacted by the cardiomyocyte is 3.5 mm or less; or
(iii) the cell density of the cardiomyocytes in the culture medium is greater than or equal to 0.625×105 cells/cm2 and less than 1.25×105 cells/cm2, and the distance from the liquid surface of the culture medium to the bottom surface of the culture vessel is 5.0 mm or less.
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