US 11,726,083 B2
Method for testing drug response of cardiomyocytes
Shinichi Sueta, Kyoto (JP); Itsunari Minami, Kyoto (JP); Tomoko Kasahara, Kyoto (JP); Kentaro Ishida, Kyoto (JP); and Naohiro Makita, Kyoto (JP)
Assigned to MYORIDGE CO. LTD., Kyoto (JP)
Appl. No. 16/957,625
Filed by Myoridge Co. Ltd., Kyoto (JP)
PCT Filed Dec. 26, 2018, PCT No. PCT/JP2018/047956
§ 371(c)(1), (2) Date Jun. 24, 2020,
PCT Pub. No. WO2019/131806, PCT Pub. Date Jul. 4, 2019.
Claims priority of application No. 2017-249552 (JP), filed on Dec. 26, 2017.
Prior Publication US 2021/0372993 A1, Dec. 2, 2021
Int. Cl. C12Q 1/02 (2006.01); G01N 33/50 (2006.01); G01N 33/84 (2006.01)
CPC G01N 33/5061 (2013.01) [G01N 33/84 (2013.01); G01N 2500/10 (2013.01)] 5 Claims
OG exemplary drawing
 
1. A method for testing drug response of cardiomyocytes, the method comprising:
(a) testing a response of the cardiomyocytes to an added drug in a culture medium under a condition in which a distance from a liquid surface of the culture medium to a bottom surface of a culture vessel contacted by the cardiomyocytes is 5.0 mm or less; or
(b) testing a response of the cardiomyocytes to an added drug immediately after placing the cardiomyocytes in a culture medium under a condition in which a distance from a liquid surface of the culture medium to a bottom surface of a culture vessel contacted by the cardiomyocytes is 5.0 mm or less, wherein
(i) the cell density of the cardiomyocytes in the culture medium is greater than or equal to 2.5×105 cells/cm2, and the distance from the liquid surface of the culture medium to the bottom surface of the culture vessel is 1.5 mm or less; or
(ii) the cell density of the cardiomyocytes in the culture medium is equal to or greater than 1.25×105 cells/cm2, and less than 2.5×105 cells/cm2, and the distance from the liquid surface of the culture medium to the bottom surface of the culture vessel contacted by the cardiomyocyte is 3.5 mm or less; or
(iii) the cell density of the cardiomyocytes in the culture medium is greater than or equal to 0.625×105 cells/cm2 and less than 1.25×105 cells/cm2, and the distance from the liquid surface of the culture medium to the bottom surface of the culture vessel is 5.0 mm or less.