CPC G01N 33/502 (2013.01) [G01N 21/6486 (2013.01); G01N 33/6896 (2013.01)] | 20 Claims |
1. A method for identifying a compound that alters fluorescence resonance energy transfer (FRET) of a protein comprising:
providing a genetically engineered cell comprising two tau proteins,
wherein a first tau protein comprises a first heterologous domain, wherein the first heterologous domain comprises a first probe,
wherein a second tau protein comprises a second heterologous domain, wherein the second heterologous domain comprises a second probe;
wherein the first and second tau proteins form an oligomer in the cell;
contacting the cell with a test compound to form a mixture; and
measuring fluorescence lifetime of the first probe, the second probe, or the combination thereof.
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