US 11,726,081 B2
Methods to identify modulators of tau protein structure
Jonathan N. Sachs, Minneapolis, MN (US); and Chih Hung Lo, Minneapolis, MN (US)
Assigned to REGENTS OF THE UNIVERSITY OF MINNESOTA, Minneapolis, MN (US)
Filed by REGENTS OF THE UNIVERSITY OF MINNESOTA, Minneapolis, MN (US)
Filed on Feb. 13, 2020, as Appl. No. 16/790,486.
Claims priority of provisional application 62/806,345, filed on Feb. 15, 2019.
Prior Publication US 2020/0264162 A1, Aug. 20, 2020
Int. Cl. G01N 33/50 (2006.01); G01N 33/68 (2006.01); G01N 21/64 (2006.01)
CPC G01N 33/502 (2013.01) [G01N 21/6486 (2013.01); G01N 33/6896 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for identifying a compound that alters fluorescence resonance energy transfer (FRET) of a protein comprising:
providing a genetically engineered cell comprising two tau proteins,
wherein a first tau protein comprises a first heterologous domain, wherein the first heterologous domain comprises a first probe,
wherein a second tau protein comprises a second heterologous domain, wherein the second heterologous domain comprises a second probe;
wherein the first and second tau proteins form an oligomer in the cell;
contacting the cell with a test compound to form a mixture; and
measuring fluorescence lifetime of the first probe, the second probe, or the combination thereof.