US 11,726,078 B2
Device for rapid detection of tuberculosis-lipoarabinomannan (TB-LAM) with enhanced sensitivity
Matthew Jeremiah Misner, Delanson, NY (US); Gregory Andrew Grossmann, Halfmoon, NY (US); Cathryn Ellen Olsen, Wilton, NY (US); John Richard Nelson, Clifton, NY (US); David Roger Moore, Rexford, NY (US); Paul Michael Smigelski, Jr., Glenville, NY (US); John Thomas Connelly, Seattle, WA (US); Benjamin David Grant, Bellevue, WA (US); and Bernhard Hans Weigl, Seattle, WA (US)
Assigned to General Electric Company, Schenectady, NY (US); and Tokitae LLC, Bellevue, WA (US)
Filed by General Electric Company, Schenectady, NY (US); and Tokitae LLC, Bellevue, WA (US)
Filed on Nov. 6, 2020, as Appl. No. 17/91,501.
Application 17/091,501 is a continuation of application No. 15/848,302, filed on Dec. 20, 2017, granted, now 10,830,760.
Prior Publication US 2021/0055282 A1, Feb. 25, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/493 (2006.01); C07K 1/22 (2006.01); B01D 15/08 (2006.01); G01N 33/50 (2006.01); G01N 33/569 (2006.01); B01D 63/02 (2006.01); A61B 10/00 (2006.01); C12M 1/26 (2006.01); G01N 33/558 (2006.01); G01N 15/14 (2006.01)
CPC G01N 33/493 (2013.01) [A61B 10/007 (2013.01); B01D 15/08 (2013.01); B01D 63/02 (2013.01); C07K 1/22 (2013.01); C12M 33/14 (2013.01); G01N 33/5038 (2013.01); G01N 33/558 (2013.01); G01N 33/569 (2013.01); G01N 33/5695 (2013.01); A61M 2202/005 (2013.01); A61M 2202/0028 (2013.01); A61M 2202/0071 (2013.01); A61M 2202/0496 (2013.01); G01N 2015/1493 (2013.01)] 22 Claims
OG exemplary drawing
 
1. A method comprising the steps of:
introducing a source biological sample to a detection device, the detection device comprising:
a pre-concentrator unit for concentrating tuberculosis lipoarabinomannan (TB-LAM) comprising:
an ion-exchange medium comprising one or more ligands configured to capture the TB-LAM from the source biological sample, wherein the captured-TB-LAM is eluted from the ion-exchange medium as an eluate comprising a concentrated form of the TB-LAM;
a cassette;
a lateral flow assay unit disposed in the cassette; and
an integration unit attached to the pre-concentrator unit and the cassette at a pivot point,
wherein the integration unit comprises a clamp to hold the pre-concentrator unit, wherein the integration unit is coupled to a lever that is adjacent to the clamp, and wherein the cassette is configured to transition from an uncoupled position to a coupled position via rotation of the integration unit about the pivot point relative to the pre-concentrator unit, wherein the pre-concentrator unit and the lateral flow assay unit disposed in the cassette are in a fluidic communication in the coupled position; and
detecting a presence of the TB-LAM in the source biological sample.