CPC G01N 33/493 (2013.01) [A61B 10/007 (2013.01); B01D 15/08 (2013.01); B01D 63/02 (2013.01); C07K 1/22 (2013.01); C12M 33/14 (2013.01); G01N 33/5038 (2013.01); G01N 33/558 (2013.01); G01N 33/569 (2013.01); G01N 33/5695 (2013.01); A61M 2202/005 (2013.01); A61M 2202/0028 (2013.01); A61M 2202/0071 (2013.01); A61M 2202/0496 (2013.01); G01N 2015/1493 (2013.01)] | 22 Claims |
1. A method comprising the steps of:
introducing a source biological sample to a detection device, the detection device comprising:
a pre-concentrator unit for concentrating tuberculosis lipoarabinomannan (TB-LAM) comprising:
an ion-exchange medium comprising one or more ligands configured to capture the TB-LAM from the source biological sample, wherein the captured-TB-LAM is eluted from the ion-exchange medium as an eluate comprising a concentrated form of the TB-LAM;
a cassette;
a lateral flow assay unit disposed in the cassette; and
an integration unit attached to the pre-concentrator unit and the cassette at a pivot point,
wherein the integration unit comprises a clamp to hold the pre-concentrator unit, wherein the integration unit is coupled to a lever that is adjacent to the clamp, and wherein the cassette is configured to transition from an uncoupled position to a coupled position via rotation of the integration unit about the pivot point relative to the pre-concentrator unit, wherein the pre-concentrator unit and the lateral flow assay unit disposed in the cassette are in a fluidic communication in the coupled position; and
detecting a presence of the TB-LAM in the source biological sample.
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