CPC G01N 31/22 (2013.01) [G01N 33/22 (2013.01)] | 15 Claims |
1. A method for detecting diesel exhaust fluid (DEF) in fuel, the method comprising:
obtaining a fuel sample to be tested;
combining the fuel sample with a buffered urease solution for making a test solution, the buffered urease solution having a pH of about 6.8 to about 7.8;
agitating the test solution;
allowing the test solution to separate into two or more layers, including an aqueous layer, and developing urea from DEF into ammonia;
adjusting a pH of the aqueous layer to greater than or equal to about 11;
contacting a reagent strip with the aqueous layer; and
developing a color change of the aqueous layer to detect a presence of ammonia.
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