CPC G01N 21/6428 (2013.01) [G01N 21/6452 (2013.01); G01N 21/6458 (2013.01); G01N 2021/6432 (2013.01); G01N 2021/6439 (2013.01)] | 28 Claims |
1. A method for selectively analyzing a sample for viable cells and nonviable cells, comprising:
providing a sample comprising one or more viable cells and/or one or more nonviable cells in a cell culture device comprising a window, the one or more viable and/or one or more nonviable cells forming or capable of forming a three-dimensional micro-tissue;
contacting the sample device, with a membrane-permeable fluorescent dye and a membrane-impermeable fluorescent quenching dye, the contacting effective to cause: the one or more viable cells and/or the one or more nonviable cells to be dyed by the membrane-permeable fluorescent dye, and the one or more nonviable cells to be dyed with the membrane-impermeable fluorescent quenching dye, the membrane-permeable fluorescent dye characterized by a first fluorescent excitation band and a first fluorescence emission band and the membrane-impermeable fluorescent quenching dye characterized by a second fluorescent excitation band and a second fluorescence emission band; and
illuminating the sample under conditions effective to cause: emitting of the first fluorescence emission band from the one or more viable cells dyed by the membrane-permeable fluorescent dye, emitting of the second fluorescence emission band from the one or more nonviable cells dyed by the membrane-impermeable fluorescent quenching dye, and quenching of at least a portion of fluorescence of the membrane-permeable fluorescent dye in the one or more nonviable cells by the membrane-impermeable fluorescent quenching dye; and
selectively analyzing the sample via the window for: a presence of the one or more viable cells according to the first fluorescence emission band and/or a presence of the one or more nonviable cells according to the second fluorescence emission band.
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