US 11,726,039 B2
Analysis of viable and nonviable cells
Ekaterina Moroz, Redwood City, CA (US); Christian Apfel, Redwood City, CA (US); and Kraig K. Anderson, Redwood City, CA (US)
Assigned to SageMedic Corporation, Redwood City, CA (US)
Filed by SageMedic Corporation, Redwood City, CA (US)
Filed on Dec. 4, 2020, as Appl. No. 17/112,263.
Application 17/112,263 is a continuation of application No. 16/600,339, filed on Oct. 11, 2019, granted, now 10,883,936.
Application 16/600,339 is a continuation of application No. 16/159,482, filed on Oct. 12, 2018, granted, now 10,564,100, issued on Feb. 18, 2020.
Prior Publication US 2021/0190688 A1, Jun. 24, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 21/64 (2006.01)
CPC G01N 21/6428 (2013.01) [G01N 21/6452 (2013.01); G01N 21/6458 (2013.01); G01N 2021/6432 (2013.01); G01N 2021/6439 (2013.01)] 28 Claims
OG exemplary drawing
 
1. A method for selectively analyzing a sample for viable cells and nonviable cells, comprising:
providing a sample comprising one or more viable cells and/or one or more nonviable cells in a cell culture device comprising a window, the one or more viable and/or one or more nonviable cells forming or capable of forming a three-dimensional micro-tissue;
contacting the sample device, with a membrane-permeable fluorescent dye and a membrane-impermeable fluorescent quenching dye, the contacting effective to cause: the one or more viable cells and/or the one or more nonviable cells to be dyed by the membrane-permeable fluorescent dye, and the one or more nonviable cells to be dyed with the membrane-impermeable fluorescent quenching dye, the membrane-permeable fluorescent dye characterized by a first fluorescent excitation band and a first fluorescence emission band and the membrane-impermeable fluorescent quenching dye characterized by a second fluorescent excitation band and a second fluorescence emission band; and
illuminating the sample under conditions effective to cause: emitting of the first fluorescence emission band from the one or more viable cells dyed by the membrane-permeable fluorescent dye, emitting of the second fluorescence emission band from the one or more nonviable cells dyed by the membrane-impermeable fluorescent quenching dye, and quenching of at least a portion of fluorescence of the membrane-permeable fluorescent dye in the one or more nonviable cells by the membrane-impermeable fluorescent quenching dye; and
selectively analyzing the sample via the window for: a presence of the one or more viable cells according to the first fluorescence emission band and/or a presence of the one or more nonviable cells according to the second fluorescence emission band.